血清学指标在兔肝纤维化模型中的实验研究

目的探讨肝纤维化血清学指标(HA、PCⅢ、LN及Ⅳ-C)与肝纤维化程度的关系。方法 45只实验兔随机分为实验组(n=36)和正常对照组(n=9)。实验组腹腔注射四氯化碳(CCl4)制作肝纤维化模型,于首次注射后第6,9,12周分批行肝纤维化血清指标检查,同时取兔肝脏行病理检查,并按病理结果分期、分级。结果共30只实验兔完成检测,其中正常肝脏9例,轻度肝纤维化6例,中度肝纤维化7例,重度肝纤维化8例。随着肝纤维化程度加重,HA、PCⅢ、LN及Ⅳ-C均逐渐增高,与肝纤维化分期之间存在正相关关系(P<0.05),其中以Ⅳ-C与纤维化分期之间关系最密切(r=0.718,P<0.05)。结论血清学指标可以反映肝纤维化的严重程度,其中以Ⅳ-C的价值较高。

Experimental study on serum fibrosis biomarkers in hepatic fibrosis rabbit model

Objective To explore the relationship between serum fibrosis biomarkers and stages of hepatic fibrosis. Methods Forty-five rabbits were randomly divided into experiment group(n=36) and control group(n=9).Hepatic fibrosis models were established by intraperitoneal injection of carbon tetrachloride(CCl4) in experiment rabbits.Serum fibrosis biomarkers(hyaluronic acid,type Ⅲ procollagen,laminin,type Ⅳ collagen) and pathology were observed respectively at the 6th,9th and 12th week after the first injection.According to pathological grade and stage,the rabbits were grouped. Results Thirty rabbits completed the detection,including 9 cases of normal liver,6 cases of mild liver fibrosis,7 cases of moderate liver fibrosis and 8 cases of severe liver fibrosis.With the aggravation of hepatic fibrosis,hyaluronic acid,type Ⅲ procollagen,laminin,type Ⅳ collagen were increased gradually.All those four biomarkers were positively correlated with histopathological staging(P<0.05),especially type Ⅳ collagen(r=0.718,P<0.05). Conclusion Serum fibrosis biomarkers are effective indices to evaluate the severity of hepatic fibrosis.Ⅳ-C is the best for the diagnosis of hepatic fibrosis in the four biomarkers.