复方乌梅散对H22荷瘤小鼠的抑瘤作用和生存期影响的研究

目的:观察复方乌梅散对小鼠移植性肝癌H22实体瘤抑制作用及生存期的影响。方法:(1)抑瘤作用观察:小鼠接种H22腹水瘤后,造成移植性小鼠肝癌模型。随机分为阴性对照组(NS组)、阳性对照组(5-FU组)、复方乌梅散水提液组、复方乌梅散醇提液组、复方乌梅散超微粉碎组,分别观察荷瘤小鼠的抑瘤率。(2)生存期观察:造模方法同前,随机分为阴性对照组(NS组)、阳性对照组(5-FU组)、复方乌梅散超微粉碎组,观察其对荷瘤小鼠生命延长率的影响。结果:(1)复方乌梅散水提液组、复方乌梅散醇提液组、复方乌梅散超微粉碎组肿瘤抑制率分别为:27.08%(P<0.05)、33.05%(P<0.05)、34.44%(P<0.05)。(2)复方乌梅散超微粉碎组生命延长时间为27.6%(P<0.05)。结论:复方乌梅散醇提液组、复方乌梅散超微粉碎组可抑制肿瘤生长,复方乌梅散超微粉碎组可有效延长小鼠生存期。

Vestigation of Anti-tumor Effects and Survival Impact of Dark Plum-Schisandra Compound on H22 Tumor-bearing Mice

Objective:To evaluate the effects of dark plum-Schisandra compound on inhibiting transplanted tumor growth and prolonging their survival time in mice with transplanted tumor H22 cells.Methods:1.To evaluate the effect on inhibiting transplanted tumor growth,mice which had got transplanted tumor H22 were randomly divided into negative control group,positive control group(5-FU),water extracts of dark plum-Schisandra compound group,ethanol extracts of dark plum-Schisandra compound group,super minute squashing extracts of dark plum-Schisandra compound group.The antitumor activity of each group was evaluated by tumor inhibitory ratio.2.To evaluate the effect on life prolonging rate,use the same way to make subcutaneous tumor model mice.Modeling mice were randomly divided into negative control group,positive control group(5-FU),super minute squashing extracts of dark plum-Schisandra compound group.Survival time of each group was evaluated.Results:(1)The tumor inhibitory rate was 27.08% in water extracts of dark plum-Schisandra compound group(P<0.05),33.05% in ethanol extracts of dark plum-Schisandra compound group(P<0.05),34.44% in super minute squashing extracts of dark plum-Schisandra compound group(P<0.05).2.The life prolonging rate of super minute squashing extracts of dark plum-Schisandra compound group is 27.6%(P<0.05).Conclusion:Ethanol extracts of dark plum-Schisandra compound group and super minute squashing extracts of dark plum-Schisandra compound group can inhibit the growth of transplanted tumor.Super minute squashing extracts of dark plum-Schisandra compound group can prolong the survival time significantly.