不同中药复方对慢性心衰大鼠MMP-2、TIMP-2影响的实验研究

目的:通过研究基质金属蛋白酶(MMP-2)及内源性基质金属蛋白酶组织抑制剂(TIMP-2)在慢性心衰中的作用,从分子生物学角度探讨中药不同配伍对慢性心衰心室重构的影响,并进一步阐明其作用机理。方法:本实验以冠脉结扎法配合力竭式游泳、减食等方法造成大鼠慢性心衰动物模型,分成模型组、西药组、益气中药组、活血中药组、益气活血中药组、益气活血利水中药组和正常组。利用实时荧光定量PCR技术及SABC免疫组化法检测慢性心衰大鼠MMP-2、TIMP-2表达的变化情况。结果:慢性心衰动物模型组大鼠心肌MMP-2表达明显升高,TIMP-2表达降低,与假手术组比较,差异有统计学意义(P<0.01);经过治疗,各用药组大鼠心肌组织MMP-2表达明显降低,TIMP-2表达升高,其中益气活血组、益气活血利水组中药与西药组比较,差异无统计学意义(P>0.05),且益气活血利水组疗效明显优于益气活血组(P<0.01)。结论:证明益气活血利水中药可通过作用于心肌组织中MMP-2、TIMP-2的表达,抑制或逆转心室重构的过程,从而达到治疗慢性心衰的目的。

Experiment Research on Effect of Different Chinese Medicine Compound on MMP-2 and TIMP-2 Expressions in Rats with Chronic Heart Failure

Objective:By investigating the effects of different Chinese medicine compounds on expressions of matrix metallopeptidase 2(MMP-2)and tissue inhibitor of metalloproteinase-2(TIMP-2)in chronic heart failure(CHF),we studied the effect of traditional Chinese medicine on further mechanism of ventricular remodeling in CHF on the role of molecular biological sense.Methods:The coronary artery ligation method with exhaustion swimming and reduce feeding was used to cause chronic heart failure rats model.Rats were divided into model group,west medicine control group,Qi-supplementing group,blood-activating group,Qi-supplementing and blood-activating group,Yiqi huoxue Lishui treatment group and normal group.The real-time quantitative PCR technique and SABC immunohistochemical method were used to detect MMP-2 and TIMP-2 changes of CHF rats.Results:MMP-2 expression in animal model of chronic heart failure group increased obviously,and TIMP-2 expression decreased,compared with the surgical group,and differences were statistically significant(P<0.01).After treatment,MMP-2 expression in the medication group significantly reduced obviously,and TIMP-2 expression increased.The difference in the supplementing Qi and activating blood group,Yiqi huoxue Lishui treatment group and Western medicine group were no statistically significant(P>0.05).And therapeutic effect of Yiqi huoxue Lishui group was much better than that in the supplementing Qi and activating blood group(P<0.01).Conclusion:It is proved Chinese compound of supplementing Qi,activating blood circulation and promoting urination can inhibit or reverse ventricular remodeling processon by acting on MMP-2 and TIMP-2 in organizations,so as to achieve the goal of therapy for chronic heart failure.