肺癌痰湿蕴肺证、气阴两虚证患者呼出气冷凝液的代谢组学研究

目的:通过分析肺癌痰湿蕴肺证和气阴两虚证患者的呼出气冷凝液(EBC)代谢产物,探讨肺癌中医证型科学客观化的可行性。方法:选择肺癌痰湿蕴肺证患者(痰湿蕴肺组)21例、气阴两虚证患者(气阴两虚组)21例及健康志愿者(健康对照组)20例,采集受试者EBC,利用气相色谱-质谱(GC-MS)技术分析EBC代谢产物。结果:痰湿蕴肺组和气阴两虚组均能够与健康对照组区分,发现9种潜在的肿瘤标志物,其中3种为痰湿蕴肺组和气阴两虚组特有化合物,6种为痰湿蕴肺组和气阴两虚组与对照组共有化合物;痰湿蕴肺组能够与气阴两虚组区分,3-氨基-2-苯甲酰基-4,5,6,7-四氢苯并[b]噻吩为痰湿蕴肺组特有化合物,1-(苯基磺酰基)吡咯为气阴两虚组特有化合物。结论:EBC的代谢组学研究方法具有用于肺癌的诊断及肺癌不同中医证型的临床客观化指标的研究的可能性。

Metabonomic Study on Exhaled Breath Condensate(EBC)of Lung Cancer with Syndrome of Turbid Phlegm Obstructing Lung and Syndrome of Deficiency of Both Qi and Yin

Objective:To analyze the exhaled breath condensate(EBC)of the patients with lung cancer of syndrome of turbid phlegm obstructing lung and syndrome of deficiency of both Qi and Yin.To explore the results of metabonomics and connotation of modern science on syndrome of turbid phlegm obstructing lung and syndrome of deficiency of both Qi and Yin in lung cancer,and try to discover biomarkers.Methods:The study included 21 patients with lung cancer of syndrome of turbid phlegm obstructing lung,21 patients with lung cancer of syndrome of deficiency of both Qi and Yin,and 20 healthy volunteers.After collection of EBC,the EBC samples were analyzed with GC-MS.Results:The patients of syndrome of turbid phlegm obstructing lung and syndrome of deficiency of both Qi and Yin were clearly distinguished from healthy persons.9 potential tumor biomarkers were discovered,including 3 molecules existed in most patient group samples,and 6 in all groups,while the quantities of the 6 molecules in patient groups were significantly different compared with healthy group.And patients of syndrome of turbid phlegm obstructing lung were able to be distinguished from those of syndrome of deficiency of both Qi and Yin.3-Amino-2-benzoyl-4,5,6,7-tetrahydrobenzo[b]thiophene existed only in patients of syndrome of turbid phlegm obstructing lung,1-benzenesulfonyl-1H-pyrrole only in patients of syndrome of deficiency of both Qi and Yin.Conclusion:The EBC analysis showed a great potential for disease diagnosis and study on Chinese medicine syndrome type.The current metabonomic method might be one of the useful measures to discover the evidence of different types of traditional Chinese medicine syndrome type.