没食子酸对人正常肝细胞LO2的体外细胞毒性研究

目的:探讨抗肿瘤单体没食子酸(GA)对正常人肝细胞(LO2)的体外细胞毒性。方法:采用MTT实验、流式细胞术、Hoechst-33258荧光染色法和电镜检测。结果:GA作用24h后,0,6.25,12.5,25,50,100,200μmol/L用药组出现不同程度的细胞增殖抑制现象,抑制率分别为0,18.2%,25.5%,42.0%,42.5%,51.8%,60.6%;流式细胞仪检测0,25,50,100μmol/L用药组凋亡率分别为1.37%,4.63%,4.64%,12.53%;荧光染色和电镜检测提示100μmol/L组细胞有形态改变,细胞核固缩、碎裂。结论:抗肿瘤单体GA对LO2细胞增殖也有抑制作用,并能诱导LO2细胞凋亡。

Research of Gallic Acid on in-vitro Cell Toxicity of Normal Human Hepatocyte LO2

Objective:To explore in-vitro cell toxicity of anti-tumor drug gallic acid(GA)on normal human hepatocyte(LO2).Methods:MTT experiment,flow cytometry,Hoechst-33258 fluorescent staining and electron microscope detection were applied.Results:0,6.25,12.5,25,50,100 and 200μmol/L medication groups presented different degrees of proliferation inhibition after GA acted on 24h,and inhibitory rates were 0,18.2%,25.5%,42.0%,42.5%,51.8% and 60.6% respectively;0,25,50 and 100μmol/L medication groups were detected by flow cytometer,whose apoptosis rates were 1.37%,4.63%,4.64% and 12.53% respectively;fluorescent staining and electron microscope detection indicated that cells manifested morphological changes,nuclear pycnosis and fragmentation in 100μmol/L group.Conclusion:Anti-tumor monomer GA also has the inhibitory effect on the proliferation of cell LO2 and can evoke LO2 apoptosis.