补肾活血中药复方对实验性糖尿病大鼠视路神经元病理损害的影响及机理探讨

目的:观察实验性糖尿病大鼠视路神经元组织形态学的病理改变,并探讨补肾活血中药复方防治实验性糖尿病大鼠视路损害的作用机理。方法:用免疫组化及Nissl染色结合图像分析半定量测定视网膜、外侧膝状体及视皮质的神经营养因子-3(neurotrophic-3,NT-3)、突触素I(synapsin I,Syn I)的表达强度和Nissl小体的含量。结果:与正常对照组相比,阴性对照组大鼠视路三级神经元的NT-3、Syn I的表达强度和Nissl小体的含量均明显降低(P<0.05或0.01);与阴性对照组相比,中药治疗组大鼠视网膜、外侧膝状体及视皮质的NT-3、Syn I表达强度和Nissl小体的含量均明显增高,尤以中药高剂量组明显(P<0.05或0.01)。结论:补肾活血中药能减轻实验性糖尿病大鼠视路三级神经元病理损害,其作用机制之一可能是通过增加糖尿病大鼠视路NT-3的表达,一定程度恢复糖尿病大鼠视觉通路神经细胞突触的密度和分布(轴突再生),并改善其神经递质释放调节功能。

Research on the Impact of Chinese Medicine for Bushenhuoxue on the Pathologic Damages of Visual Pathway in Experimental Diabetid Rats and its Mechanism

Objective:To investigate the influence of traditional Chinese medicine(TCM) for bushenhuoxue on the pathologic damages of visual pathway in experimental diabetid rats.Methods:The model of experimental diabetic rats was induced by streptozotocin(STZ).Expression of neurotrophic-3 synapsin I and the content of Nissl bodies in retina,external geniculate body(EGB)and visual cortex(VC)were shown by immunohistochemical staining and toluidine blue-eosin staining,and were quantitated by image analysis system.Results:Compared with the normal control group,the positive staining of neurotrophic-3 Syn I and the content of Nissl bodies in retina,EGB and VC were significangtly low in negative control group(P<0.05 or 0.01);Compared with the negative control group,the positive staining of neurotrophic-3 Syn I and the content of Nissl bodies in retina,EGB and VC were significangtly high in all of the TCM groups,especially in the high dose group(P<0.05 or 0.01).Conclusion:The experimental diabetic rats which were in high blood glucose condition for 6 months,the optic pathway neuron had the varying degree of the recessive change;TCM of bushenhuoxue could increase the expression of neurotrophic-3 and synapsin I,accordingly it may be one of the important mechanisms of TCM for bushenhuoxue to relieve the damages of neurons in visual pathway with diabetic rats.