清肠化湿方对TNBS诱导大鼠UC模型紧密连接蛋白的影响

目的:观察三硝基苯磺酸(TNBS)法诱导大鼠溃疡性结肠炎模型结肠黏膜occludin、claudin-1蛋白的变化特点及清肠化湿方对occludin、claudin-1蛋白表达的影响。方法:采用TNBS诱导大鼠溃疡性结肠炎模型,并随机分为正常对照组、模型对照组、清肠化湿方组、SASP组,治疗10天后处死大鼠取新鲜结肠标本,观察黏膜大体形态及组织学改变,并采用Western blot法检测occludin、claudin-1蛋白表达水平。结果:模型对照组occludin、claudin-1蛋白表达均明显低于正常对照组,清肠化湿方组occludin、claudin-1的表达高于模型对照组,同时,清肠化湿方组大鼠结肠形态及组织学损伤评分均有降低。结论:清肠化湿方对UC模型大鼠具有治疗作用,增强occludin、claudin-1的表达,恢复正常的肠粘膜屏障功能可能是其作用机制之一。

Effects of Qingchang Huashig Decoction on Tight Junction Proteins in TNBS-induced Ulcerative Colitis Model Rats

Objective:To observe the effect of Qingchang Huashig Decoction(QD)on the protein expressions and change character of occludin and claudin-1 in colonic mucosa of rats with ulcerative colitis(UC).Methods:Rat UC model induced by trinitrobenzene-sulfonic acid(TNBS)were randomly divided into normal group,model group,QD group and SASP group.The rats were sacrificed to get their colonic mucosa and extract the whole-cell protein after 10d of treatment.Western blot was performed to detect the protein levels of occludin and claudin-1.Results:The relative protein expressions of occludin and claudin-1 in the model group were all lower than those in the normal group significantly.The relative protein expressions of occludin and claudin-1 in the QD group were higher than that in the model group.The colonic configurative and histological injury score were declined.Conclusion:Qingchang Huashig Decoction can treat ulcerative colitis.One of the mechanisms may be that Qingchang Huashig Decoction can increase the relative protein expressions of occludin and claudin-1 and restore the normal intestinal mucosal barrier function.