同病异证H22肝癌小鼠肿瘤组织细胞凋亡通路基因差异表达的特征

目的:观察昆明种H22肝癌小鼠邪毒壅盛证与气虚证肿瘤组织细胞凋亡通路基因表达特征,探讨中医同病异证发生的物质基础。方法:采用小鼠计量化辨证方法,筛选出瘤早期邪毒壅盛证与气虚证同病异证H22肝癌小鼠,采用Affymetrix GeneChip Mouse Exon 1.0 ST Array检测肿瘤组织细胞凋亡通路相关基因表达的特征。结果:依据KEGG数据库细胞凋亡通路,筛选出芯片数据中匹配的33个基因,发现(1)TNF-α和IL-1依赖的细胞凋亡信号通路总体上不利于邪毒壅盛证小鼠肿瘤细胞的凋亡;(2)IL-3依赖的细胞凋亡信号通路总体上反似有利于邪毒壅盛证小鼠肿瘤细胞凋亡;而(3)线粒体介导的细胞凋亡信号通路,邪毒壅盛证小鼠一致表达下调,提示不利于其细胞凋亡。结论:邪毒壅盛肝癌小鼠肿瘤细胞恶性增殖程度高,可能与线粒体介导的细胞凋亡信号通路系列相关基因表达轻度抑制有关。

Genes Different Expression in Tumor Apoptosis Pathway of H22 Liver Cancer Mice with Different Symptoms

Objective:To observe genes expression in tumor cell apoptosis pathway of Kunming H22 liver cancer mice of poisonous pathogenic factors syndrome and asthenia of qi syndrome and probe the material basis of the same disease with different symptoms.Methods:Apply measurement of the syndrome differentiation method in mice.Screen poisonous pathogenic factors syndrome and asthenia of qi syndrome with H22 liver cancer in mice and use Affymetrix GeneChip Mouse Exon 1.0 ST Array to detect tumor cells apoptosis pathway-related gene expression characteristics.Results:According to the KEGG database apoptosis pathway,the data filtered out 33 matched genes in the chip.(1)TNF-α-dependent and IL-1-dependent apoptosis pathway in general was not conducive to tumor cell apoptosis for poisonous pathogenic factors syndrome.(2)IL-3-dependent apoptotic pathway in general seems to be favorable for tumor cell apoptosis for poisonous pathogenic factors syndrome.(3)In the mitochondria-mediated apoptosis signaling pathway,poisonous pathogenic factors syndrome downregulated,suggesting negative effect on their apoptosis.ConclusionThe high degree of malignant proliferation with poisonous pathogenic factors syndrome may be related to the mild inhibition of gene expression in mitochondria-mediated apoptosis signaling pathway.