四君子汤对肺癌恶病质小鼠骨骼肌萎缩及部分炎症细胞因子的影响

目的:观察四君子汤对肺癌恶病质小鼠骨骼肌萎缩的影响,并从炎性细胞因子的角度对其部分作用机制进行探讨。方法:利用lewis肺癌细胞株皮下接种C57B1/6小鼠,建立肺癌恶病质小鼠模型。将60只小鼠随机分为6组,荷瘤中药长期大剂量组,荷瘤中药短期大剂量组,荷瘤中药长期小剂量组,荷瘤中药短期小剂量组,荷瘤空白组,生理盐水对照组。监测各组小鼠的去瘤体重和腓肠肌重量,测量血清TNF-α和IL-6水平。结果:肺癌恶病质小鼠腓肠肌重量明显下降(P<0.05),血清TNF-α和IL-6水平明显升高(P<0.05),治疗组可以不同程度地缓解恶病质小鼠腓肠肌重量的下降,其中以长期大剂量组效果最优(P<0.05)。结论:四君子汤能够减缓肺癌恶病质小鼠骨骼肌萎缩的发生程度,其作用机制可能与减少部分炎性细胞因子的表达有关。

Effect of Sijunzi Decoction on Skeletal Muscle Atrophy and Some Inflammatory Cytokines in Lung Cancer Cachexia Mice

Objective:To observe the effect of Sijunzi Decoction on skeletal muscle atrophy in lung cancer cachexia mice,and to discuss the mechanism from inflammatory cytokines.Methods:To establish mice model of cancer cachexia by inoculating lewis lung cancer cell lines to C57B1/6 mice subcutaneously.60 mice were randomly divided into 6 groups.The tumor weight and gastrocnemius muscle weight were monitored,and the serum tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)levels were measured.Results:The weight of gastrocnemius muscle were significantly decreased in the mice(P<0.05),serum TNF-α and IL-6 levels were significantly increased(P<0.05).In treated groups,the weight reduction of gastrocnemius in cachexia mice and the serum levels reduction of TNF-α and IL-6 were slight.Conclusion:Sijunzi Decoction could slowdown the cancer cachexia of skeletal muscle atrophy,and its mechanism may be associated with the decreasing expression of inflammatory cytokines.