动态观察柴胡皂苷a对实验性癫痫小鼠痫性发作的影响

目的研究柴胡皂苷a(saikosaponin a,SSa)对戊四氮(PTZ)诱导的小鼠痫性发作的影响,了解其药效特点.方法采用PTZ诱导小鼠急性痫性发作来制作癫痫模型,通过记录不同时间点小鼠痫性发作潜伏期和强直性惊厥率的变化,研究SSa抗小鼠PTZ致痫作用的动态特征.结果 SSa 腹腔注射30、60、90 min组能显著延长阵发性痉挛潜伏期(P<0.05),其中SSa 90 min组作用最为显著(P<0.01).在60、90、120 min组中,SSa 腹腔注射也能明显延长强直性惊厥潜伏期(P<0.05),其中尤以90 min组作用显著(P<0.01);SSa 90 min组对过渡时间延长作用显著(P<0.05);SSa 30 min、60 min组能减少PTZ致痫小鼠强直性惊厥发作例数(P<0.05),且以SSa 60 min组作用最为显著.结论 SSa具有对抗小鼠PTZ致痫作用;腹腔注射SSa对PTZ诱导小鼠的痫性发作在120 min内以60 min、90 min药效作用较强.

Dynamic observation of the effect of saikosaponin a on mice with experimental seizure

Objective To study the dynamic variations of the effect of saikosaponin a(SSa) on epileptic mice induced by pentylenetetrazol(PTZ),and to understand the effect characteristics of SSa.Method With the epileptic mice induced by PTZ as animal model.In the different time point,the tentative seizure incubation period and the case of tetanic convulsion were recorded to study the characteristics of the antiepileptic effect of SSa on mouse induced by PTZ.Results In the group of 30min,60 min and 90 min,the incubation period of clonic cramp was significantly prolonged by SSa(P<0.05),and in the group of 90 min,the effect was remarkable(P<0.01).Also,intraperitoneal injection of SSa could prolong the incubation period of tetanic convulsion in the group of 60 min,90 min and 120 min(P<0.05).The effect was especially evident in the group of 90 min(P<0.01).In the group of 90 min,the transition time was remarkably prolonged by SSa(P<0.05).In the group of SSa 30 min and SSa 60 min,SSa could reduce the tetanic convulsion of epileptic mice introduced by PTZ(P<0.05),and the effect is more significant in the group of 60min than that in the group of 30min.In the group of 30 min and 60 min,SSa could protect the epileptic mice introduced by PTZ from tetanic convulsion more significantly than those in the group of 90 min and 120 min.Conclusions SSa can inhibit the epileptic mice induced by PTZ,SSa can protect the mouse from tetanic convulsion,and in 2 hours,the effect was more remarkable at 60th min,90th min than others.