饮水有机致突物染毒小鼠病理学变化与p53突变

探讨肿瘤机体常见的基因变化是否也常见于啮齿类动物模型。「方法」研究饮水有机致突物染毒实验小鼠的Ｐ５３基因突变。「结果『病理组织分析发现各实验组小鼠肝、肾有明显的病理变化。用ＰＣＲ－ＳＳＣＰ方法分析：高剂量组Ｐ５３基因点突变检出率（９／１０）高于对照组（３／１１）。各样本病理学与Ｐ５３基因分结果差异无显著性。

Study on the Pathological Changes and the Mutations in the p53 Gene in Mice Treated with Organic Mutants in Drinking Water

Purpose] To explore whether common genetic events in human tumor also oceur in rodent models. [Methods]The presence of p53 mutation in mice treated with organic mutants extracted from the drinking water of household was investigated. [Results] Obvious histo-pathological changes of the liver and kidney tissues of the studied mice were observed. The frapuency of point mutations of the p53 gene in high group (9/10) was higher than that in the negative control group (3/11 ) by polymerase chain reaction single -strand conformation polymorphism analysis. No significant association was observed between the results of pathological analysis and the detection of the p53 gene for each Sample. [Conclusion] The results suggest that the DNA extracted from paraffin embedded tissue may be ussd to detect the mutation of the p53 gene, also can evaluate retrospectively the toxicity of organic mutants in the drinking water in mice. So the detection method Of the point mutation of p53 gene will be a supplementary as pathological analysis to judge the damages of tissues instudied animals.