慢性HIV-1感染人群血浆不同中和能力及与有关因素的研究

目的 探讨安徽省既往献血的HIV慢性感染人群血浆中和能力及其与CD4、病毒载量的关系.方法 从安徽省既往献血的294位HIV慢性感染人群静脉抽血,EDTA抗凝后进行血浆和外周血单个核细胞(PBMC)的分离、CD4和病毒载量的检测,采用拥有共享序列的临床分离病毒1597株和实验室株SF33病毒两种病毒分别做血浆的中和试验.用200TCID50病毒感染TZM-bl细胞,通过加入不同稀释度血浆,用Luciferase Assay System检测血浆的中和能力并计算中和50%的病毒进入细胞的血浆浓度(EC50).结果 294位患者血浆针对HIV-1实验株SF33和临床分离株1597的中和能力间差异有统计学意义(P<0.05),针对实验株的中和能力明显高于临床分离株,91%患者血浆对HIV-1实验株SF33具有一定的中和抗体,其中EC50>500的抗体水平较高的血浆占33%,对临床分离株1597的中和抗体阳性率为79%,其中EC50>500的占7%.方差分析显示,血浆中和能力在CIM不同水平之间没有显著差异.294位HIV-1B'亚型感染的血浆病毒载量(1g)平均为4.40(2.60～6.46).方差分析显示,血浆对SF33的中和能力在不同病毒载量水平之间有显著差异,病毒载量不同的各组血浆针对1597病毒的中和能力之间差异无统计学意义.经Spearman's B相关分析,对SF33株的中和水平与病毒载量呈正相关,对1597株的中和水平与病毒载量的相关性没有统计学意义.在病毒载量1g<4的患者中,10.53%血浆样品的EC50高于500;在病毒载量1g≥5的患者中,6.46%血浆样品的EC50高于500.结论 HIV-1慢性感染人群的血浆对实验株SF33的中和能力随着病毒复制能力的提高而增加,但对临床分离株1597的中和能力未见相关性.

Viral load and neutralizing activity of plasma from HIV-1 chronic infected former blood donors in Anhui province, China

Objective To explore the association among viral load,CD4 count and neutralizing ac-tivity of plasma from chronic HIV-1 infected former blood donors in Anhui province, China. Methods 294 chronically HIV-1 B' infected individuals from former blood donors in Anhni province were enrolled, whose plasma and peripheral blood mononuelnar cells (PBMC) were isolated. The viral load and CD4 were detec-ted, and the neutralization activity of plasma against primary virus (1597) and lab-adapted strain (SF33) was detected by Luciferase Assay System based on TZM cell line, and 50% neutralizing level was calculated by the Reed-Mueneh method. Results Neutralizing activity responding against SF33 strain was higher than 1597 (P<0.05). There was a positive correlation between neutralizing concentration of plasma against SF33 and viral load (1g) (r = 0.191, P = 0.001), but there was no correlation between neutralizing concen-tration of plasma against 1597 and viral load(lg) (r = 0.097, P = 0.096). Conchtsion In chronic HIV-1 infectious people, neutralizing level against SF33 increases with viral replication, however, there is no asso-ciation of plasma neutralizing against 1597 replication with viral load.