乳腺癌缺失基因1对肝癌生物学行为的影响

目的探讨乳腺癌缺失基因1（deleted in breast cancer 1,DBC1）对肝癌细胞增殖和凋亡的影响。方法构建pcDNA3.1-DBC1的正义表达载体,转染肝癌细胞SMMC-7721,MTT法检测DBC1对肝癌细胞SMMC-7721生长增殖的影响,采用流式细胞术（FCM）检测DBC1对肝癌细胞SMMC-7721的细胞周期和细胞凋亡的影响。结果与转染空载体的SMMC-7721细胞相比,pcDNA3.1-DBC1正义表达载体转染上调DBC1的表达,可显著抑制肝癌细胞（SMMC-7721）增殖,升高G1期细胞比例（51.0%vs 64.9%,P=0.002）,降低S期细胞比例（29.7%vs 14.0%,P〈0.001）,诱导细胞凋亡（8.8%vs 24.6%,P〈0.001）。结论 DBC1通过抑制肿瘤细胞增殖,阻滞细胞周期进展,促进细胞凋亡,抑制肝癌的发生和发展。

Effect of DBC1 on biological behavior of liver cancer

Objective To study the effect of deleted genes of breast cancer 1(DBC1) on proliferation and apoptosis in liver cancer cells.Methods pcDNA3.1-DBC1 vector was constructed and transfected into human liver cancer cell line SMMC-7721.MTT assay and flow cytometry were performed to detect the effect of DBC1 on the proliferation,cycle,and apoptosis of cells.Results Compared with cells transfected with the control vector,entropic expression of DBC1 gene with pcDNA3.1-DBC1 vector significantly inhibited SMMC-7721 proliferation.Entropic expression of DBC1 caused a significant increase in the percentage of cells in the G1 phase(51.0% vs 64.9%,P=0.002),a significant decrease in the percentage of cells in the S phase(29.7% vs 14.0%,P<0.001),and induced cell apoptosis(8.8% vs 24.6%,P<0.001)in SMMC-7721 cells.Conclusions DBC1 can inhibit the development and progression of hepatic carcinoma by suppressing cell growth and causing cell cycle arrest and cell apoptosis.