Analysis of two strains of <Emphasis Type="Italic">Peanut stunt virus</Emphasis>: satRNA-associated and satRNA free |
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Authors: | Aleksandra Obrępalska-Stęplowska Marta Budziszewska Przemysław Wieczorek Anna Czerwoniec |
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Institution: | (1) Interdepartmental Laboratory of Molecular Biology, Institute of Plant Protection—National Research Institute, Poznan, Poland;(2) Bioinformatics Laboratory, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland |
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Abstract: | Peanut stunt virus (PSV) is a pathogen of legumes, vegetables, trees, and weeds occurring worldwide. The species is characterized by significant
genetic variability. PSV strains are classified into four subgroups on the basis of their nucleotide sequence homology. Here,
we are presenting two further, fully sequenced PSV strains—PSV-Ag and PSV-G, that could be considered as I subgroup representatives.
However, their sequence homology with other typical I subgroups members, similarly as another strain—PSV-P, characterized
by our group previously, is lower than 90%. This lead us to propose further subdivision of the I subgroup into IA, IB, and
IC units, and to classify PSV-Ag and PSV-G strains to the last one. In this article, we are showing that identity level of
PSV-Ag and PSV-G is very high and apart from the presence of satRNA in the first one, they differ only by a few nucleotides
in their genomic RNAs. Nevertheless, symptoms they cause on host plants might differ significantly, just as the levels in
infected plants. Effect of single amino acid changes between strains on the three-dimensional structure of viral proteins
was analyzed. Differences occur mainly on the protein surfaces which can possibly affect protein–protein interaction in infected
cells, which is discussed. |
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