含氯消毒剂对新型冠状病毒核酸破坏效果研究

目的观察含氯消毒剂对新型冠状病毒核酸的破坏效果,评估实时荧光定量PCR技术用于新型冠状病毒消毒效果评价的可行性。方法采用悬液定量灭活试验法,观察不同浓度含氯消毒剂、不同作用时间对新型冠状病毒的灭活效果,使用荧光定量PCR方法检测新型冠状病毒核酸进行消毒效果评价。结果1000 mg/L和2000 mg/L有效氯消毒剂作用30 s以上就能破坏新型冠状病毒核酸。750 mg/L有效氯消毒剂作用15 min以上能破坏新型冠状病毒核酸。500 mg/L有效氯消毒剂作用30 min不能完全破坏新型冠状病毒核酸。结论国家防控方案规定的使用1000 mg/L有效氯对新型冠状病毒疫点作用30 min的消毒方法,可以完全破坏新型冠状病毒核酸。可以使用实时荧光定量PCR技术用于新型冠状病毒消毒效果评价。

Study on the effect of chlorine disinfectants on the destruction of New coronavirus nucleic acid

Objective To observe the destructive effects of chlorine disinfectants on the nucleic acid of novel coronavirus,and to assess the feasibility of real-time fluorescent quantitative PCR technology for the evaluation of the disinfection effect of novel coronavirus.Methods A suspension quantitative inactivation test was used to observe the inactivation effects of different concentrations of chlorine containing disinfectants and different action times on the novel coronavirus.RT-PCR method was used to detect novel coronavirus nucleic acid for the disinfection effect evaluation.Results The chlorine disinfectants of 1000 mg/L and 2000 mg/L could destroy the nucleic acid of ew coronavirusafter application for over 30 s.The chlorine disinfectant of 750 mg/L could destroy the nucleic acid of new coronavirusafter application for over 15 min.Chlorine disinfectant of 500 mg/L could not completely destroy the nucleic acid of new coronavirusafter 30 min action.Conclusion The disinfection method stipulated in the national prevention and control plan using 1000 mg/L effective chlorine for the epidemic sites of new coronavirus for 30 minutes could completely destroy the nucleic acid of new coronavirus.The Real-time quantitative PCR can be used to evaluate the disinfection effect of new coronavirus.