HAD对创伤失血性休克大鼠肝细胞线粒体功能的保护作用

目的 探讨高渗醋酸酐（ＨＡ／Ｄ）对大鼠创伤失血性休克后肝细胞线粒体功能的影响。方法 大鼠６４只，随机分为正常对照组、休克末组、生理盐水（ＮＳ，４ｍｌ．ｋｇ＾－１体重）治疗后３０、６０、１２０ｍｉｎ组及ＨＡＤ（４ｍｌ．ｋｇ＾－１体重）治疗后３０、６０、１２０ｍｉｎ组，每组８只，股骨骨折加股动脉放血制作创伤失血性休克模型，各 运行至实验时限后活杀，取肝组织测定测定细胞线粒体呼吸控制率（ＲＣＲ）、腺苷二

Effects of hypertonic sodium acetate/dextran on hepatocytic mitochondria during traumatohemorrhagic shock in rats

Objective To study the effects of hypertonic sodium acetate/dextran (HA/D) on hepatocytic mitochondria during traumatohemorrhagic shock in rats. Methods 64 rats were randomized into normal control, normal saline (NS) treated and HA/D treated groups. The animal model of traumatohemorrhagic shock was established with fracture of the femur followed with bleeding through the femoral artery. Respiratory control rate (RCR), ADP/O, H + ATPase and NADH CoQ reductase activity and calcium concentration in hepatocytic mitochondria were determined 30, 60 and 120 minutes after the administration of NS or HA/D. Results RCR, ADP/O and the activity of H + ATPase and NADH CoQ reductase of hepatocytic mitochondria were more markedly improved in HA/D treated group than in NS treated group and calcium concentration of hepatocytic mitochondria was markedly lower in the formar than in the latter. Conclusion Infusion of small volume of HA/D can significantly attenuate the dysfunction of hepatocytic mitochondria in traumatohemorrhagic shock in rats.