野菊花超声辅助提取工艺优选

目的:优选野菊花提取工艺.方法:以总黄酮、绿原酸和蒙花苷含量为指标,采用超声辅助及正交试验优选野菊花水提和醇提工艺,并与常规回流提取进行比较;采用UV测定总黄酮含量,RP-HPLC测定绿原酸和蒙花苷含量.结果:综合3个指标,最佳水提工艺为加15倍量水提取2次,每次30 min,提取温度75℃;最佳醇提工艺为15倍量80％乙醇提取30 min,提取温度70℃.结论:采用醇提工艺提取野菊花时,总黄酮、绿原酸和蒙花苷得率均较高,可为野菊花综合开发提供实验依据.

Optimization of Ultrasound-assisted Extraction Technology for Chrysanthemum indicum

Objective:To optimize extraction technology of Chrysanthemum indicum.Method: With the content of total flavonoids,chlorogenic acid and linarin as indexes,water extraction and ethanol extraction technology were optimized by ultrasound-assisted and orthogonal test,and compared with conventional reflux extraction;The content of total flavonoids was determined by UV,the content of chlorogenic acid and linarin were determined by RP-HPLC.Result: In total of three indexes,optimum water extraction technology was as follows:Adding 15 folds of water and extracting for 2 times,30 min each time,extraction temperature 75 ℃;optimum alcohol extraction process was as follows: extracted 30 min with 15 folds the amount of 80% ethanol,extraction temperature 70 ℃.Conclusion: Total flavonoids,chlorogenic acid and linarin were relatively high with alcohol extraction technology for C.indicum;it could provide experimental basis for comprehensive development and utilization of C.indicum.