| RP-HPLC测定藏成药十味手参散中胡椒碱的含量 |
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| 引用本文： | 陈海娟,纪兰菊. RP-HPLC测定藏成药十味手参散中胡椒碱的含量[J]. 中国实验方剂学杂志, 2012, 18(13): 122-125 |
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| 作者姓名： | 陈海娟 纪兰菊 |
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| 作者单位： | 1. 青海师范大学生命与地理科学学院,青藏高原环境与资源教育部重点实验室,西宁810008
2. 中国科学院西北高原生物研究所,西宁,810008 |
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| 摘 要： | 目的:建立藏成药十味手参散中胡椒碱的含量测定方法.方法:色谱条件Kromasil-C18 (4.6 mm×250 mm,5μm),流动相甲醇-水(77∶ 23),检测波长343 nm,流速1.0 mL· min-1.结果:胡椒碱在0.448～2.24 μg呈良好线性关系(r=0.9995),十味手参散中胡椒碱的平均回收率为99.43％(n=6).结论:所建立的方法简便、快速、准确、灵敏度高、重复性好,可有效控制藏成药十味手参散的质量.
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| 关 键 词： | 十味手参散 胡椒碱 反相高效液相色谱法 |
| 收稿时间： | 2011-11-16 |
Content Determination of Piperine in Shiwei Shoushen Powder by RP-HPLC |
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| CHEN Hai-juan and JI Lan-ju. Content Determination of Piperine in Shiwei Shoushen Powder by RP-HPLC[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(13): 122-125 |
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| Authors: | CHEN Hai-juan and JI Lan-ju |
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| Affiliation: | School of Life and Geographical Science, Qinghai Normal University, Key Laboratory of Education Department of Environment and Resources on Qinghai-Tibetan Plateau, Xining 810008, China;Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China |
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| Abstract: | Objective:To establish a feasible method for the content determination of piperine in Shiwei Shoushen powder by RP-HPLC.Method:An HPLC method was adopted with column Kromasil-C18(4.6 mm×250 mm,5 μm).The mobile phase was consisted of methanol-water(77∶23).The flow velocity was 1.0 mL·min-1.The detection wavelength was at 343 nm.Result:The calibration curve showed a good linearity with in the range of 0.448-2.24 μg.The average recovery was 99.43%.Conclusion:This method is convenient,rapid and reliable for quality control of this preparation. |
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| Keywords: | Shiwei Shoushen powder piperine RP-HPLC |
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