HPLC同时测定糖敏灵丸中5种成分的含量

目的:建立HPLC同时测定糖敏灵丸中芍药内酯苷、芍药苷、柚皮苷、新橙皮苷及黄芩苷含量的方法.方法:采用Aglient ZorbaxSB-C18色谱柱(4.6 mm × 250 mm,5μm),以0.05％磷酸水溶液-乙腈为流动相进行梯度洗脱,流速1 mL·min-1,检测波长230 nm,柱温30℃.结果:芍药内酯苷、芍药苷、柚皮苷、新橙皮苷及黄芩昔的线性范围分别为0.008～0.320,0.021 ～0.832,0.088 ～3.52,0.124～4.96,0.086 ～3.44 μg(r≥0.999 9,n=7);平均回收率(n=6)分别为99.7％( RSD2.0％),100.1％( RSD 1.8％),100.1％( RSD 1.5％),100.0％ (RSD2.4％),103.6％( RSD 2.4％).结论:所建立的HPLC方法简便,结果准确、可靠,重复性好.

Simultaneous Determination of Five Components in Tangminlingwan by HPLC

Objective:To establish an HPLC method for simultaneous detemination of albiflorin,paeoniflorin,naringin,neohesperidin and baicalin in Tangminlingwan.Method: The chromatographic method was carried out on an Aglient Zorbax SB-C18 column(4.6 mm×250 mm,5 μm) with acetonitrile-0.05% phosphoric acid solution as the mobile phase by gradient elution.The flow rate was 1 mL·min-1.The detection was set at the wavelength of 230 nm.The column temperature was at 30 ℃.Result: The linear ranges of albiflorin,paeoniflorin,naringin,neohesperidin and baicalin were 0.008-0.320,0.021-0.832,0.088-3.52,0.124-4.96 and 0.086-3.44 μg(r≥0.999 9,n=7),respectively.The average recoeries were 99.7%(RSD 2.0%),100.1%(RSD 1.8%),100.1%(RSD 1.5%),100.0%(RSD 2.4%) and 103.6%(RSD 2.4%),respectively.Conclusion: The method is simple,accurate and reproducible.