白金妇安颗粒中白芍和柴胡提取工艺优选

目的:优选白金妇安颗粒中白芍和柴胡的提取工艺.方法:以HPLC测定芍药苷提取量,分光光度法测定的柴胡总皂苷提取量,采用单因素试验法优选提取溶剂,正交试验法对白金妇安颗粒提取工艺条件进行优选.结果:以70％乙醇为溶剂时芍药苷苷提取量最多,乙醇体积分数＞50％时,柴胡总皂昔提取量基本不变；优选提取工艺白芍、柴胡粉碎成粗粉,分别加8,6倍量70％乙醇回流提取2次,每次2h.结论:优选工艺合理,有效成分提取率高.

Optimization of Extraction Process for Paeonia lactiflora and Bupleurum chinense from Baijin Fuan Granule

Objective: To optimize extraction process of Paeonia lactiflora and Bupleurum chinense from Baijin Fuan granule. Method: With extraction volume of paeoniflorin and total saponins from B. chinense as indexes which were determined by HPLC,extraction solvent was optimized by single-factor test;With the content of paeoniflorin and total saponins from B. chinense as indexes, extraction process of Baijin Fuan granule was optimized by orthogonal test. Result: Extraction volume of paeoniflorin was the most with 70% ethanol as solvent,but when the concentration of ethanol was more than 50%,total saponins from B. chinense was basically unchanged;Optimized extraction technology was P. lactiflora and B. chinense were crushed into coarse powder, refluxing extracted 2 times with 8,6 times the amount of 70% ethanol,respectively.2 h per time. Conclusion: Optimized process was reasonable with high yield of active ingredients.