野菊花多糖对小鼠免疫功能低下的保护作用

目的:研究野菊花多糖对环磷酰胺所致小鼠免疫功能低下的保护作用.方法:将小鼠随机分为正常对照组、模型组及野菊花多糖高、低剂量组,每组10只.野菊花多糖高、低剂量组分别ig给药400,200 mg·kg-1·d-1,连续14 d；于ig的第10天开始,模型组及野菊花多糖高、低剂量组分别经腹腔注射环磷酰胺100 mg·kg-1 ·d-1,连续4d,制备免疫功能低下小鼠模型 .观察野菊花多糖对免疫功能低下小鼠碳廓清试验的影响；测定小鼠体重及胸腺、脾脏质量,计算胸腺、脾指数；采用二硝基氟苯诱导小鼠迟发型变态反应法(耳肿胀法)测定细胞免疫功能；检测血清溶血素含量.结果:与正常对照组比较,模型组小鼠的各项指标均显著降低(P＜0.01).野菊花多糖高、低剂量组的碳廓清指数分别为0.027 8±0.005 9,0.019 9±0.004 7,吞噬指数分别为5.397 0±0.735 6,4.827 8±0.480 1,耳肿胀度分别为(5.23±0.98),(4.89±1.10) mg,血清溶血素分别为(0.410±0.063),(0.357±0.058).模型组的碳廓清指数为0.0134±0.0038,吞噬指数为4.231 6±0.367 2,耳肿胀度为(3.21±0.91)mg,血清溶血素吸光度(A)为0.299±0.032.各指标与模型组比较,均具有显著性差异( P＜0.01或P＜0.05).结论:野菊花多糖可增强环磷酰胺所致免疫功能低下小鼠的免疫功能.

Regulatory Effect of Chrysanthemum indicum Polysaccharide on Immune Function in Mice with Hypoimmunity

Objective: To study the protective effect of the Chrysanthemum indicum polysaccharide(CIP) on immunosuppressed mice caused by cyclophosphamide. Method: The mice were randomly divided into four groups (10 in each group): normal control group, model group and CIP groups (high and low dosage). CIP high and low dosage groups were administered intragastrically with CIP at dose of 400, 200 mg·kg^-1, respectively once daily for fourteen days. At the tenth day model group and CIP groups were administered with cyclophosphamide by peritoneal injection for four days to induce an immunosuppressed mouse model. The effect on carbon clearance was observed. The weight of the mice,thymus and spleen was measured; the thymus index and spleen index were calculated. The cell immune function was assessed by delayed type hypersensitivity induced by dinitrofluorobenzene in normal mice. The serum level of hemolysin was determined. Result: All the indexes in the model group were reduced(P<0.01). The carbon clearance rate in CIP high and low dosage groups was 0.0278±0.0059 and 0.0199±0.0047 respectively, the phagocytic exponent was 5.3970±0.7356 and 4.8278±0.4801, ear edema was (5.23±0.98) mg and (4.89±1.10) mg, the serum level of hemolysin was 0.410±0.063 and 0.357±0.058. The carbon clearance rate in model group was 0.0134±0.0038,the phagocytic exponent was 4.2316±0.3672, ear edema was (3.21±0.91)mg,the serum level of hemolysin was 0.299±0.032.Compared with model group, all was significantly increased (P<0.01 orP<0.05). Conclusion: CIP can improve the non-specific, humoral and cellular immune functions in cyclophosphamide-induced immunosuppressed mice.