二参汤对异丙肾上腺素致大鼠心肌缺血损伤的保护作用

目的:通过观察二参汤对心肌缺血大鼠血清学和组织学相关指标的影响,探讨二参汤抗心肌缺血的机制.方法:将健康Wistar大鼠用随机数字表法分为5组,二参汤高、中、低剂量组(100,50,20 g·kg-1),空白对照组和模型组,每组10只.二参汤连续ig 14 d后ip注射5 mg·kg-1异丙肾上腺素(ISO)造成心肌缺血性损伤模型后观察心电图标Ⅱ导联变化,血清血栓素A2( TXA2)、前列环素(PGI2)含量的变化及对心肌细胞凋亡相关基因Bax mRNA,Bcl-2 mRNA的表达的影响.结果:与模型组相比,二参汤组在注射ISO后5～30 min各时间段均见T波明显回落(P＜0.05)；二参汤组均可不同程度降低血清TXA2的含量,二参汤高剂量组(102.16± 9.41) μg· L-1显著低于模型组( 188.81±38.70) μg·L-1(P＜0.05),增加血清PGI2的含量,二参汤高剂量组(112.10±10.45)μg·L-1显著高于模型组(36.10±4.26)μg·L1(P＜0.05),调整TXA2/PGI2的平衡；二参汤能抑制心肌组织Bax mRNA的表达,上调Bcl-2 mRNA的表达(P＜0.05),保持Bcl-2 mRNA/Bax mRNA的平衡.结论:二参汤通过凋节PGI2/TXA2的平衡,从而抑制血管内皮炎症性改变,保护血管内皮功能,发挥抗心肌缺血作用,通过调节Bcl-2mRNA/BAXmRNA的平衡,调整细胞凋亡基因平衡,抑制过度凋亡保护心肌细胞,达到抗心肌缺血作用.

Protective Effect of Ersheng Decoction on Myocardial Ischemia Induced by Isoproterenol in Rats

Objective:The effect of Ersheng decoction on myocardial ischemia in rats.Method: Wistar rats was randomly divided into 5 groups,Ersheng decoction high dose group,middle dose group,low dose group(100,50,20 g·kg-1),control group and model group,ten rats in each group.The changes of electrocadiograph(ECG) II lead,serum level of thromboxane A2(TXA2),prostacylin(PGI2) and the content of Bax mRNA,Bcl-2 mRAN on myocardial cells were detected.Result: Compared with model control group,Ersheng decoction group decreased the T wave(P<0.05),and could reduce the blood serum content of TXA2,which was significantly lower in Ersheng decoction high dose group(102.16±9.41)μg·L-1 than that in model group(188.81±38.70)μg·L-1(P<0.05),increase the serum PGI2 content,which was significantly higher in Ersheng decoction high dose group(112.10±10.45)μg·L-1 than that in model group(36.10±4.26) μg·L-1(P<0.05);Ersheng decoction could inhibit Bax mRNA expression,up-regulation of Bcl-2 mRNA expression(P<0.05).Conclusion: Ersheng decoction can protect myocardial cells against myocardial ischemia injury,which may be related to regulating expression of Bcl-2 mRNA and Bax mRNA.