新藤黄酸对S180细胞株的体内外抗肿瘤作用

目的:评价新藤黄酸对S180肿瘤细胞株的体内外抗肿瘤作用.方法:将不同浓度的新藤黄酸分别作用于体外培养的S180细胞48 h,用CCK-8法检测药物对S180细胞的增殖抑制作用；以8.0,4.0,2.0 mg·kg-1剂量ip给予S180腹水瘤小鼠,每天1次,连续7d,观察45 d对存活时间的影响；以16.0,8.0,4.0 mg·kg-1剂量ig给予荷S180实体瘤小鼠,每天1次,连续12d,评价新藤黄酸的体内抗肿瘤作用；测单次ip给药对小鼠的LD50.结果:新藤黄酸对体外培养S180细胞的增殖有明显的抑制作用,药物作用48 h其IC50为(1.54±0.12) mg·L-1；4.0 mg· kg-1新藤黄酸ip给药可使S180腹水瘤小鼠存活时间较荷瘤对照组延长141.6％,新藤黄酸对S180实体瘤的抑制作用随着剂量的增大而升高,并呈一定剂量依赖性,体内抗肿瘤显著(P＜0.05)；单次ip给药对小鼠的LD50为36.66 mg·kg-1.结论:新藤黄酸对S180细胞株具有明显的体内外抗肿瘤作用.

Anti-tumor Effects of Neogambogic Acid on S180 Cell Line Both in vivo and in vitro

Objective: To evaluate the anti-tumor effects of neogambogic acid on S180 cell line both in vivo and in vitro. Method: CCK-8 method was used to assay the anti-proliferative effect of neogambogic acid with different concentrations for 48 h on S180 cell line in vitro. The anti-tumor effect in vivo was evaluated by the survival time of the mice bearing S180 ascitic tumor, which was ip treated with 8.0, 4.0, 2.0 mg·kg^-1 neogambogic acid respectively, once per day for continuous 7 days, and observation period lasted for 45 days, and the mice bearing S180 xenograft was ig treated with 16.0, 8.0, 4.0 mg·kg^-1 neogambogic acid respectively, once per day for continuous 12 days. The LD₅₀ of mice was determined based on single administration (ip)of different doses of neogambogic acid. Result: Neogambogic acid showed obvious anti-proliferative effect on S180 cell culture, the IC₅₀ of 48 h was (1.54±0.12) mg·L^-1. And the in vivo test showed that the survival time of the mice administrated with 4.0 mg·kg^-1 neogambogic acid was prolonged 141.6%. The inhibitory effects of the mice bearing tumor increased with the increasing of the concentration of neogambogic acid in a dose-dependent manner (P<0.05). The LD₅₀ of neogambogic acid (ip) was 36.66 mg·kg^-1. Conclusion: The results suggest that the anti-tumor effect of neogambogic acid on S180 cell line is significant both in vivo and in vitro.