| UV测定广西不同产地倒地铃中总黄酮的含量 |
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| 引用本文： | 陈君,韦建华,李兵,蔡少芳,张明站. UV测定广西不同产地倒地铃中总黄酮的含量[J]. 中国实验方剂学杂志, 2012, 18(13): 105-107 |
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| 作者姓名： | 陈君 韦建华 李兵 蔡少芳 张明站 |
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| 作者单位： | 1. 柳州医学高等专科学校,广西柳州,545005
2. 广西中医学院,南宁,530001 |
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| 基金项目： | 广西壮族自治区自然科学基金(2011GXNSFB018103) |
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| 摘 要： | 目的:建立紫外分光光度法测定广西不同产地倒地铃全草总黄酮的含量.方法:以芦丁为对照品,以亚硝酸钠-硝酸铝-氢氧化钠为显色剂,测定波长为488 nm.结果:芦丁在0.009 35 ～0.056 0 g·L-1与峰面积具有良好的线性关系(r=0.999 3)；平均加样回收率为100.05％(n=6),RSD 2.3％.结论:该法准确、简便、稳定可靠,可用于倒地铃中总黄酮的含量测定.
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| 关 键 词： | 倒地铃 芦丁 总黄酮 紫外分光光度法 含量测定 |
| 收稿时间： | 2011-07-01 |
Determination of Contents of Total Flavonoids in Cardiospermum halicacabum by UV |
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| CHEN Jun,WEI Jian-hu,LI Bing,CAI Shao-fang and ZHANG Ming-zhan. Determination of Contents of Total Flavonoids in Cardiospermum halicacabum by UV[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(13): 105-107 |
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| Authors: | CHEN Jun WEI Jian-hu LI Bing CAI Shao-fang ZHANG Ming-zhan |
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| Affiliation: | Liuzhou Medical School, Liuzhou 545005, China;Guangxi University of Traditional Chinese Medical, Nanning 530001, China;Guangxi University of Traditional Chinese Medical, Nanning 530001, China;Guangxi University of Traditional Chinese Medical, Nanning 530001, China;Guangxi University of Traditional Chinese Medical, Nanning 530001, China |
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| Abstract: | Objective:To establish the methods of determination on the contents of total flavonoids in Cardiospermum halicacabum by UV.Method:Rutin was used as reference substance and sodium nitrite-nitrate aluminum-sodium hydroxide as color-showing reagent,measurement wavelength was set at 488 nm.Result: The linear response range was 0.009 35-0.056 0 g·L-1(r=0.999 3).The average recovery was 100.05%,RSD 2.3%(n=6).Conclusion: The method was simple,accurate and repeoducible which could be used for determination of the total flavonoids in C.halicacabum. |
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| Keywords: | Cardiospermum halicacabum rutin total flavonoids ultraviolet spectro-phometry determination |
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