| bFGF对局灶性脑缺血大鼠脑组织ERK表达的影响 |
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| 引用本文： | 吕威力,邢雪松,赵海.bFGF对局灶性脑缺血大鼠脑组织ERK表达的影响[J].解剖学研究,2011,33(2). |
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| 作者姓名： | 吕威力 邢雪松 赵海 |
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| 作者单位： | 1. 沈阳医学院病理学教研室,辽宁,沈阳,110034
2. 沈阳医学院人体解剖学教研室 |
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| 基金项目： | 辽宁省教育厅科学研究计划项目 |
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| 摘 要： | 目的研究大鼠脑缺血再灌注后对胞外信号调节激酶(extracellular signal-regulated kinase,ERK)表达的影响,探讨碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)对脑组织缺血再灌注神经元ERK的调节作用及机制。方法应用线栓法制作大鼠局灶性脑缺血再灌注模型,大脑中动脉阻塞2 h再灌注损伤24 h,采用TUNEL法、免疫组化检测海马及皮质内神经元凋亡和ERK的表达。结果 sham组鼠海马及大脑皮质偶见凋亡细胞,大脑皮质及海马神经元内少见ERK免疫反应阳性细胞;I/R组鼠海马及皮质神经元凋亡增加,缺血再灌注损伤后大脑皮质及海马神经元内ERK阳性表达明显高于假手组;bFGF组鼠海马及皮质神经元凋亡减少,皮质及海马神经元内ERK表达较I/R组明显增加。结论 bFGF显著减少缺血神经元凋亡,上调脑缺血诱导的ERK表达,对脑缺血再灌注海马及皮质神经元的具有保护作用。
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| 关 键 词： | 脑缺血 碱性成纤维细胞生长因子 外信号调节激酶 海马 凋亡 |
Effects of bFGF on the expression of ERK in cerebral tissue during focal cerebral ischemia in rats |
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| LV Wei-li,XING Xue-song,ZHAO Hai.Effects of bFGF on the expression of ERK in cerebral tissue during focal cerebral ischemia in rats[J].Anatomy Research,2011,33(2). |
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| Authors: | LV Wei-li XING Xue-song ZHAO Hai |
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| Institution: | LV Wei-li,XING Xue-song,ZHAO Hai.Department of Pathology,Shenyang Medical College,Shenyang 110034,China |
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| Abstract: | Objective To investigate the expression of ERK in hippocampus and cortex after cerebral ischemia and reperfusion in rats,explore the regulative effects and mechanism of basic fibroblast growth factor(bFGF)to ERK on brain tissue.Methods The model of middle cerebral arteries occlusion(MCAO) were performed with intraluminal filament blockade.The expression of ERK and apoptosis cell in hippocampus and cortex were detected with immunohistochemical method and TUNEL method.Results No apoptotic cells were observed ... |
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| Keywords: | Cerebral ischemia bFGF Extracellular signal-regulated kinase Hippocampus Apoptosis |
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