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骨髓间充质干细胞向淋巴管内皮细胞的分化潜能
引用本文:白英伟,田兴松.骨髓间充质干细胞向淋巴管内皮细胞的分化潜能[J].中国现代普通外科进展,2011,14(5):344-349.
作者姓名:白英伟  田兴松
作者单位:山东大学附属省立医院乳腺和甲状腺外科,山东济南,250021
摘    要:目的:观察骨髓间充质干细胞在体外分化成淋巴管内皮细胞的可能性。方法:采用密度梯度离心法分离成人骨髓间充质干细胞,体外扩增,流式细胞术检测细胞表面标志。用内皮细胞培养基EGM-2培养,加入VEGF-C(156s)诱导后,相差显微镜下观察细胞形态变化,流式细胞术检测淋巴管内皮细胞特异性标志物Podoplanin和VEGFR-3,RT-PCR检测相应基因的表达。成管实验检测分化的淋巴管内皮细胞的功能。结果:分离培养的骨髓间充质细胞形态上为纤维样,经流式细胞术测定表达CD90、CD105,其阳性表达率分别为90.03%、97.86%,而CD14、CD34、HLA-DR的阳性阳性率分别为2.00%、2.18%、1.56%。经诱导刺激后,间充质干细胞呈鹅卵石样外观,Podoplanin和VEGFR-3的表达率分别为26.25%和12.70%。RT-PCR结果显示特异性的条带,成管实验显示诱导后的淋巴管内皮细胞具有成管的功能。结论:从骨髓中可以提取到骨髓间充质干细胞,在内皮细胞培养基和VEGF-C(156s)的诱导下骨髓间充质干细胞可以分化为淋巴管内皮细胞。

关 键 词:骨髓间充质干细胞  淋巴管内皮细胞  细胞培养  细胞分化

Differentiation potential of bone marrow mesenchymal stem cells into lymphatic endothelial cells
BAI Ying-Wei,TIAN Xing-Song.Differentiation potential of bone marrow mesenchymal stem cells into lymphatic endothelial cells[J].Chinese Journal of Current Advances in General Surgery,2011,14(5):344-349.
Authors:BAI Ying-Wei  TIAN Xing-Song
Institution:BAI Ying-Wei,TIAN Xing-Song Department of Breast and Thyroid Surgery,Provincial Hospital Affiliated to Shandong University(Jinan 250012,China)
Abstract:Objectives: To explore the differentiation potential of bone marrow mesenchymal stem cells(MSCs) into lymphatic endothelial cells in vitro.Methods: MSCs were isolated from human bone marrow use Ficoll density gradient centrifugation.After amplification MSCs were cultured in Endothelial cell growth medium-2 with VEGF-C(156s).RT-PCR and flow cytometry were used to characterize the MSCs and lymphatic endothelial cells.tubulogenesis formation assay was performed to verified the fuction of the new lymphatic endo...
Keywords:Mesenchymal stem cell·Lymphatic endothelial cell·Cell culture·Cell differentiation
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