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| HPLC法测定尿毒清颗粒中大黄酸的含量 | |
| 引用本文： | 何洁英,王汝上,黄振山. HPLC法测定尿毒清颗粒中大黄酸的含量[J]. 现代医院, 2011, 11(6): 30-31. DOI: 10.3969/j.issn.1671-332X.2011.06.014 |
| 作者姓名： | 何洁英 王汝上 黄振山 |
| 作者单位： | 1. 佛山市第一人民医院,广东佛山,528000 2. 广州康臣药业有限公司肾病药物研究中心,广东广州,510530 3. 卫材(中国)药业有限公司,广东佛山,528000 |
| 摘 要： | 目的建立高效液相色谱法测定尿毒清颗粒中大黄酸含量的方法。方法采用HPLC法,色谱柱:Phenomenex(250 mm×4.6 mm,5μm),流动相:甲醇-0.1%磷酸溶液(85∶15),柱温:室温,流速:1.0 ml.min-1,检测波长:254 nm。结果大黄酸在0.834 5μg.ml-1～25.035μg.ml-1内呈良好线性,R2=0.999 6,平均回收率为100.34%,RSD为1.06%(n=9)。结论本方法准确、精密度高、专属性好,可用于尿毒清颗粒的质量控制。 |
| 关 键 词： | 高效液相色谱法 大黄酸 尿毒清颗粒 含量测定 |
DETERMINATION OF THE CONTENTS OF RHEIN IN NIAODUQING GRAINS BY HPLC |
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| HE Jieying,WANG Rushang,HUANG Zhenshan. DETERMINATION OF THE CONTENTS OF RHEIN IN NIAODUQING GRAINS BY HPLC[J]. Modern Hospital, 2011, 11(6): 30-31. DOI: 10.3969/j.issn.1671-332X.2011.06.014 | |
| Authors: | HE Jieying WANG Rushang HUANG Zhenshan |
| Affiliation: | HE Jieying,WANG Rushang,HUANG Zhenshan The First People's Hospital of Foshan City,Guangdong Province 528000 PRC |
| Abstract: | Objective To determine the contents of Rhein in Niaoduqing grains by means of the HPLC（High Performance Liquid Chromatography）.Methods A HPLC has been established by the mobile phase of methanol-0.1% H_3PO_4 aqueous solution for Rhein in Niaoduqing grains,using a Phenomenex column（250 mm×4.6 mm,5 μm）.Detect wavelength was 254 nm,column temperature was room temperature,flow rate was 1 ml·min-1 and injection volume was 10 μl.Results The celibration curve was linear in the range of 0.834 5 μg·ml-1～25.035 μg·ml-1 for Rhein,with R2 of 0.999 6,the average recovery of 100.34% with the RSD of 1.06%.Conclusion The method is reliable,accurate and specific,which can be used for the quality control of Niaoduqing grains. |
| Keywords: | HPLC Rhein Niaoduqing grains Determination of content |
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