| B-7.1转基因细胞株的表达和检测 |
| |
| 引用本文： | 刘岩,王科亮,杨德军. B-7.1转基因细胞株的表达和检测[J]. 解剖学研究, 2011, 33(2): 92-94 |
| |
| 作者姓名： | 刘岩 王科亮 杨德军 |
| |
| 作者单位： | 哈尔滨医科大学附属第四医院泌尿外科,黑龙江,哈尔滨,150001 |
| |
| 基金项目： | 黑龙江省教育厅科学技术项目 |
| |
| 摘 要： | 目的 构建B-7.1基因真核表达载体并导入CAK-1肾细胞癌细胞表达,为进一步研究基因的功能做材料准备.方法 采用RT-PCR方法,以正常肝脏组织cDNA为模板,扩增B-7.1基因全长读码框架,并构建到真核表达载体pCDNA3.1中,将其导入CAK-1肾细胞瘤细胞,对转染后的细胞进行RT-PCR和Western blo...
|
| 关 键 词： | B-7.1 肾细胞癌 转基因 表达 载体 |
Expression of human B-7.1 transgene cell line and detection of the expressing pattern |
| |
| LIU Yan,WANG Ke-liang,YANG De-jun. Expression of human B-7.1 transgene cell line and detection of the expressing pattern[J]. Anatomy Research, 2011, 33(2): 92-94 |
| |
| Authors: | LIU Yan WANG Ke-liang YANG De-jun |
| |
| Affiliation: | LIU Yan,WANG Ke-liang,YANG De-jun.Department of Urology,The Fourth Affiliated Hospital of Harbin Medical University,Harbin 150001,China |
| |
| Abstract: | Objective Construction of B-7.1 eukaryotic expression vector and import CAK-1 expression in renal cell carcinoma,for further study of gene function to do material preparation.Methods By RT-PCR method,regarding normal liver tissue cDNA as a template,B-7.1 gene is amplified full-length reading frame.And then construct the eukaryotic expression vector pCDNA3.1,import it to CAK-1 renal cell carcinoma cells,The transfected cells was identified by RT-PCR and Western blot analysis.Results the recombinant was verif... |
| |
| Keywords: | B-7.1 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
