复方浙贝颗粒联合阿霉素对K562／A02移植瘤细胞凋亡及相关蛋白表达的影响

本研究旨在探讨复方浙贝颗粒联合阿霉素对K562／A02移植瘤细胞凋亡及BCL-2、BAX蛋白表达影响。将多药耐药K562／A02细胞接种于BALB／c—nu裸鼠腋前皮下构建多药耐药移植瘤模型，用Annexin V—FITC／PI流式细胞术检测实验各组移植瘤细胞的凋亡率；用免疫组织化学染色法检测实验各组移植瘤细胞的BCL-2、BAX蛋白表达，并计算蛋白表达积分的光密度值（IOD）。结果发现，复方浙贝颗粒各剂量联合阿霉素组的细胞凋亡率与对照组、阿霉素组相比有统计学意义（P〈0．05）；与对照组和阿霉素组相比，复方浙贝颗粒各剂量联合阿霉素组多药耐药移植瘤BCL-2表达的IOD值降低（P〈0．05），BAX表达的IOD值增高（P〈0．05）。结论：复方浙贝颗粒是通过增强阿霉素对耐药移植瘤的促细胞凋亡，减少耐药移植瘤的BCL-2蛋白表达，增加BAX蛋白表达而逆转多药耐药。

Effect of Compound Zhe-Bei Granule(CZBG)Combined with Doxorubicin on Cell Apopotosis and Expression of Related Proteins in K562/A02 Multidrug Resistance Tumor Xenografts in Mice

This study was purposed to investigate the effects of compound Zhe-Bei granule （CZBG） combined with doxorubicin （Dox） on the expression of BCL-2, BAX and cell apopotosis rate in K562/A02 multidrug resistant tumor xenografts. Tumor xenograft model was established by injecting the K562/A02 cells into the axillary flank of BALB/c nude mice. The cell apoptosis rate of xenografts was detected by flow cytometry with Annexin V-FITC/PI staining. The expressions of BCL-2 and BAX in xenografts were measured by immunohistochemistry staining. The value of integral optical density （IOD） was calculated according to staining image on slide. The results indicated that as compared with normal saline control group and Dox group, the high, middle, low dose of CZBG combined with Dox could increase the cell apoptosis rate of tumor xenografts with statistical significance （p 〈 0.05 ） ; and could decrease the IOD value of BCL-2 and increase the IOD value of BAX in xenografts with statistical significance （p 〈 0.05 ）. It is concluded that CZBG combined with Dox can enhance the apoptosis rate in K562/A02 cells, down-regulate the expression of BCL-2 and up-regulate the expression of BAX, thereby, can reverse the multidrug resistance of K562/A02 cells by decreasing the ratio of BCL-2/BAX.