大鼠输尿管梗阻后肾炎中的P-选择素表达及意义

目的　探讨粘附分子P-选择素在单侧输尿管梗阻后肾炎中的作用。方法　建立单侧输尿管梗阻(UUO)动物模型，观察肾组织病理改变和P-选择素在肾组织中的表达，以及抗P-选择素单克隆抗体(单抗)对其影响。结果　大鼠UUO第1d即见间质以单核巨噬细胞为主的炎细胞浸润和p-选择素在小管间质表达；第3d间质炎细胞增多，P-选择素明显表达；第7d间质炎细胞浸润达高峰伴间质水肿，而P-选择素表达下降。抗P-选择素单抗治疗组炎细胞浸润减轻，P-选择素表达减弱。结论　 P-选择素在UUO早期参与介导以单核巨噬细胞为主的炎细胞在肾间质浸润及肾间质损害，抗P-选择素单抗对此具有一定治疗作用。

Significance of P- selectin Expression in Unilateral Ureteral Obstruction

Objective To determine the relationship between P-selectin and unilateral ureteralobstruction (UUO). Methods The effect of anti - P - selectin monoclonal antibody (P - selectinmAb) on the renal histopathological changes and P- selectin expression was investigated in the UUO ex-perimental model. Results Renal interstitial macrophage infiltration was observed within 24h afterUUO, with marked increase at day 3 and a peak response at day 7. Interstitial edema was also found atday 7. The expressin of P- selectin was upregulated on the tubular epithelium and within the intersti-tium as early as 24h after ureteral ligation, with a peak expression at day 3 and down- regulated at day7. Macrophage accumulation was significantly inhibited when P- selectin was blocked by mAb.Conclusion P- selectinplays a role in interstitial macrophage infiltration in the early phase of UUO,and P- selectin mAb might improve the interstitial leisons.