首页 | 本学科首页   官方微博 | 高级检索  
检索        

荷胃癌小鼠自杀基因治疗后宿主免疫功能观察
引用本文:顾琴龙,纪玉宝,姬舒荣,郭善禹,林言箴.荷胃癌小鼠自杀基因治疗后宿主免疫功能观察[J].上海交通大学学报(医学版),2000,20(6).
作者姓名:顾琴龙  纪玉宝  姬舒荣  郭善禹  林言箴
作者单位:上海第二医科大学瑞金医院 上海消化外科研究所,上海 200025
基金项目:卫生部科研项目,上海市自然科学基金
摘    要:目的 研究自杀基因疗法是否能诱导肿瘤宿主产生免疫应答。方法将小鼠前胃癌细胞株(MFC)接种于小鼠背部制作肿瘤模型,以单纯疱疹病毒胸苷激酶基因(HSV-tk)和大肠杆菌胞嘧啶脱氧基酶基因(CD)注射于瘤体内,并于瘤体周围注射mIL-2和mGM-CSFDNA及向腹腔内注射前药9-丙氧乌苷(GVC)和5-Fc。治疗第5周取肿瘤消退小鼠的脾细胞测自然杀伤细胞(NK)和LAK活性;第12周取PBL和脾细胞测NK和细胞毒细胞(CTL)活性。结果在TK/CD基因联合细胞因子及前药治疗后第5周,实验组脾细胞NK和LAK活性均明显高于荷瘤鼠对照(P<0.01)。12周后,实验组PBL的NK和CTL活性均显著高于正常对照组(P=0.05,P<0.01);而脾细胞NK活性与对照组无显著差异(P>0.05)。结论自杀基因疗法能较长时间提高宿主抗肿瘤免疫反应。

关 键 词:胃肿瘤  基因治疗  肿瘤免疫

A Study on the Immune Response of Mice with Gastric Cancer Induced by Gene Therapy
Abstract:Objective To study whether the suicide gene therapy can induce the immune re-sponse of the tumor bearer. Methods The tumor model was made by inoculating MFC on the back ofthe mice. HSV- tk gene and CD gene were injected into the tumor; mIL-2; mGM-CSF DNA wereinjected around the tumor; prodrugs(GVC and 5 - Fc) were injected into the abdominal cavity. In thefifth week after gene treatment, the NK and LAK activities of the spleen cells from the tumor bearerwere determined. In the twelfth week, the NK and CTL activities of PBL or spleen cells were tested.Results In the fifth week after gene therapy was administered, the NK and LAK activities of the spleencells from the gene therapy experimental group were obviously higher than tumor bearer controls( P < 0.01). In the twelfth week of treatment, the NK and CTL activities of PBL from experimental mice areenhanced markedly to compare with normal controls(NK, P = 0.05; CTL, P<0.01). There is no dif-ference in the NK activity of the spleen cells between experimental group and control group(P > 0.05).Conclusion The suicide gene therapy can enhance the antitumor immunity ability for a rather long time.
Keywords:gastric neoplasm  gene treatment  tumor immunity
本文献已被 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号