| 急性白血病P16基因mRNA表达水平的研究 |
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| 引用本文： | 程丽芳,杨玲,尹列芬,尚学琴,瞿晓媛.急性白血病P16基因mRNA表达水平的研究[J].云南医药,2008,29(3). |
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| 作者姓名： | 程丽芳 杨玲 尹列芬 尚学琴 瞿晓媛 |
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| 作者单位： | 昆明医学院第二附属医院血液科,云南昆明650101 |
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| 摘 要： | 目的研究P16基因mRNA的表达水平与急性白血病发生发展及预后的相关性。方法采用荧光实时定量逆转录聚合酶链反应（RT-PCR）技术检测50例急性白血病患者P16基因mRNA表达水平。结果急性白血病患者P16基因mRNA表达水平明显低于正常人mRNA表达水平（P〈0.001）。其中急性淋巴细胞白血病mRNA表达水平又低于急性髓细胞性白血病（P〈0.05）。初诊组、难治复发组、初诊组、完全缓解组与正常对照组mRNA表达水平依次升高（P〈0.05）。初诊组中完全缓解组mRNA表达水平高于未完全缓解组（P〈0.001）。结论P16基因表达缺陷在急性白血病的发生、发展中起重要作用,且与白血病患者的治疗和预后有一定关系。
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| 关 键 词： | P16基因mRNA 急性白血病 荧光实时定量RT-PCR法 |
The expression of P16 gene's mRNA in acute leukemia. |
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| CHENG Li-fang,YANG Ling,YIN Lie-feng,et al..The expression of P16 gene''s mRNA in acute leukemia.[J].Medicine and Pharmacy of Yunnan,2008,29(3). |
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| Authors: | CHENG Li-fang YANG Ling YIN Lie-feng |
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| Institution: | CHENG Li-fang,YANG Ling,YIN Lie-feng,et al.(Hematology Department,Second Affiliated Hospital,Kunming Medical College,Kunming,Yunnan 650101,China) |
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| Abstract: | Objective To investigate the relationship between the pathogenesis,development and prognosis of acute leukemia and the expression of P16 gene. Methods RT-PCR methods was used to detect mRNA expression of P16 gene in acute leukemia. Results The results showed that the expression of P16 in AL group was strikingly lower than that in control one. Expression of P16 gene in ALL was lower than that in AML. Normal control group was the highest,relapse group was lowest. Expression of P16 gene showed statistical sign... |
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| Keywords: | P16 gene Acute leukemia Real-time quantitative RT-PCR |
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