布鲁氏菌非典型菌株及R型菌株鉴定分类的研究

本文报告了用布氏菌常规鉴定法、六大群噬菌体裂解试验和钒化代谢捡查对17株非典型及R型菌株进行综合鉴定分类研究;并建立和采用4株布氏菌系列McAb和S型牛、羊、猪种布氏菌的OMP的SDS-PAGE图谱用于非典型布氏菌分类之中。
通过实际鉴定菌株证明,采用常规鉴定方法、六大群噬菌体裂解分甩和氧化代谢试验的综合鉴定可以对大多数非典型和R型菌株定种定型。
首次对利用布氏菌系列McAb和布氏菌OMP图谱鉴定非典型及R型菌株进行探索,并为利用这两种方法鉴定这两类菌株的可能性提供了依据。

A Study on Identification of Atypical and R Phase Strains of Brucella

This paper reports a comprehensive study on taxonomy of Brucella. In which 17 atypical and R phase strains were identified by using the routine methods, 6 groups of phages and oxidative metabolic tests. 4 McAb of Brucella were developed and applied for identification. Meanwhile OMP profiles of S phase of B. abortus, B. melitensis and B. suis were compared during identification of them. The results show that a comprehensive identification with routine methods, phage-typing and oxidative metabolic tests are able to determine these strains if they are species or biotypes. Several strains of Brucella McAb and OMP profiles of Brucella were firstly applied for identification of atypical and R strains, and provided some possibility in the application of identification of Brucella by using these two methods.