脑缺血再灌注后神经元特异性烯醇化酶和S-100β蛋白变化与神经功能等级评定的关系

背景脑脊液和血浆中神经元特异性烯醇化酶(NSE)和神经组织蛋白S-100β含量在脑缺血性损伤时升高的程度能反映脑损伤的程度和预后.目的观察大鼠脑缺血再灌注损伤脑组织NSE和S-100β的变化规律及其意义.设计随机对照的研究.地点及对象本实验在青岛大学医学院脑血管病研究所和山东省脑血管病防治重点实验室完成.成年健康雌性SD大鼠36只,体质量230～270g,清洁级,由中国科学院上海实验动物中心提供.方法实验由作者完成.方法用线栓法建立大鼠大脑中动脉缺血再灌注模型,应用神经等级评分观察脑缺血再灌注后行为功能的恢复,免疫组织化学法检测脑组织中NSE和S-100β的动态变化.主要观察指标各组大鼠皮质区、纹状体NSE,S-100β含量及神经功能评分.结果皮质区、纹状体区的NSE和S-100β免疫阳性反应均于缺血再灌注后12h明显增强,并随时间变化而逐渐下降,至14 d降至正常水平.神经功能评分再灌注2h～1 d时为3.00分,再灌注3,7,14 d恢复至1.50分(t=2.60～4.48,P＜0.05).结论脑缺血再灌注损伤后脑组织NSE和S-100β蛋白表达增加,并与神经功能恢复有相关性.

Relationship between the changes of neuron specific enolase and S-100β protein following cerebral ischemia-reperfusion and neural functional grading

BACKGROUND:The contents of neuron-specific enolase (NSE) and nerve tissue protein S-100β in cerebrospinal fluid and plasma will increase after cerebral ischemic injury. The increments of NSE and S-100β can reflect the severity and the prognosis of cerebral ischemia.OBJECTIVE: To observe the changing patterns of NSE and S-100β after the damage to the cerebral tissues caused by cerebral ischemia reperfusion in rats and its corresponding significance.DESIGN:A randomized controlled basic research.SETTING AND SUBJECTS: This experiment was carried out at the Institute of Cerebrovascular Diseases, the College of Medicine, Qingdao University and the Key Laboratory of Cerebrovascular Diseases of Shandong Province. Thirty-six adult healthy female SD rats, weighing 230- 270 g,cleaning grade, were purchased from Shanghai Experimental Animal Center of the Chinese Academy of Science.METHODS: The experiment was conducted by the authors. The models of ischemia reperfusion in SD rats were established by middle cerebral artery occlusion (MCAO) with a nylon monofilament suture. Neurological functional grading evaluation was performed on the same rats to examine the behavioral recovery. Immunohistochemical technique was used to investigate the dynamic changes of NSE and S-100β contents in cerebral tissues.MAIN OUTCOME MEASURES: The outcome measures include the contents of NSE and S-100β in cortex and striatum, neural functional scoring.RESULTS: The peak content of NSE occurred 12 hours after reperfusion,and decreased gradually with the time, and then decreased to the normal level on day 14. The scores of neurological function were 3.00 at 2 h - 1 d after reperfusion, and recovered to 1.50 at 3, 7, 14 days after reperfusion (t =2.60-4.48, P ＜0.05)CONCLUSIONS: The contents of NSE and S-100β increased significantly in the cerebral tissue after cerebral ischemia reperfusion, and it was correlated with the recovery of neural functions.