孕酮对牙周膜成纤维细胞c—los、c-jun基因表达的影响

目的：研究孕酮对牙周膜成纤维细胞c—fos、c-jun基因表达的影响，探讨孕酮促进人牙周膜成纤维细胞（hPDLCs）增殖和成骨分化的可能机制。方法：原代培养hPDLCs，取第4-6代细胞用于实验。分为空白对照组，孕酮组，抑制剂组。各组细胞培养2h，采用反转录聚合酶链反应检测c—fos和c-junmRNA的表达。结果：RT—PCR的结果说明，孕酮能促进c—fos和c-junmRNA的表达，抑制剂组c—fos和c-junmRNA的表达下调。结论：孕酮能够上调hPDLCs中c—fos、c-jun的表达。提示孕酮可能通过上调c—fos、c-jun的表达促进hPDLCs的增殖和成骨分化，促进骨形成。

Effects of progesterone on the expressions of c-fos and c-jun mRNA in periodontal ligament cells.

Objective：To study the effects of progesterone on the expressions of c-fos and c-jun mRNA in hPDLCs and explore the potential mechanism that progesterone promotes the proliferation and differentiation of hPDLCs. Method：The hPDL tissue was obtained from the extracted healthy premolar for orthodontic reasons. The hPDLCs used in this study under- went four to six passages. Cells were untreated or treated with 100 nM progesterone in the absence or presence of 100 nM RU486. The expressions of c-fos and c-jun mRNA were determined by RT-PCR. Result：The results showed that the expres- sions of c-fos and c-jun mRNA were markedly enhanced in 100 nM Progesterone-treated group compared with control group,but decreased in progesterone＋RU486 group. Conclusion：Progesterone can upregulate the expressions of c-fos and c- jun mRNA. Our current study suggests that progesterone may exert its bone-sparing functions by increasing the expression level of c-fos and c-jun mRNA via PR in hPI）LCs.