羧甲基壳聚糖对LPS干预人牙周膜成纤维细胞表达IL-6的影响

目的：脂多糖（LPS）干预下，不同浓度羧甲基壳聚糖（CMC）对人牙周膜成纤维细胞（HPDLFs）表达白细胞介素-6（IL-6）的影响。方法：取冻存的HPDLFs进行复苏培养，以浓度为10mg／L的LPS进行诱导，用不同浓度的羧甲基壳聚糖进行干预，采用免疫组织化学方法检测IL-6在HPDLFs中的表达变化。结果：不同浓度羧甲基壳聚糖能够下调同一浓度LPS诱导的HPDLFs表达的IL-6，随羧甲基壳聚糖浓度的增加，IL-6的表达量呈逐渐减弱趋势（P〈0．05）。结论：羧甲基壳聚糖可能对LPS诱导的HPDLFs炎症损伤过程中表达IL一6有重要的抑制作用，为牙周病治疗提供新的实验资料。

Effect of carboxymethyl chitosan on the expression of IL-6 in human periodontal ligament fibroblasts induced by LPS.

Objective：To explore effects of different dosage of carboxymethyl chitosan （CMC） combined with lipopolysaccharide （LPS） on expression of IL-6 in human periodontal ligament fibroblasts （HPDLFs）. Method： Take cryopre- served HPDLFs to cultur.The HPDLFs were induced by 10 mg / L LPS combined with different dosage of CMC. Then the ex- pression levels of IL-6 protein were observed in cultured HPDLFs by immunohistochemistry. Result： CMC was able to down- regulate the expression of IL-6 in the cultured HPDLFs induced by the LPS and the regulation was dosage-dependent.The declining trend of IL-6 expression in the cultured LPS-induced HPDLFs was obviously following the increasing CMC dosage addition. Conclusion： The carboxymethyl chitosan may play an important role in inhibiting the expression of IL-6 in LPS- induced HPDLFs, which provides new scientific information for the clinical treatment of periodontal disease.