骨髓间充质干细胞通过血管内皮细胞生长因子受体2调控神经干细胞的增殖与分化

目的 观察血管内皮细胞生长因子受体2(VEGFR2)在骨髓间充质干细胞(MSCs)调控神经干细胞(NSCs)增殖与分化中的作用.方法 细胞共培养分为5组:NSCs+MSCs、NSCs+MSCs+SU5416(VEGFR2阻断剂)、NSCs+HMVECs、NSCs+HMVECs+SU5416、NSCs+3T3.共培养6 h后,采用逆转录.聚合酶链反应检测各组NSCs标志物nestin、成熟星形胶质细胞标志物GFAP及Notch信号分子Notchl和hesl的表达.结果 在MSCs及HMVECs共培养组中nestin的表达较高,当VEGFR2信号通路被阻断后,nestin的表达明显下降,而成熟神经细胞GFAP的表达却明显增加.同时,在MSCs及HMVECs共培养组中,Notchl、hesl的表达也明显高于3T3对照组及VEGFR2信号通路阻断剂组.结论 VEGFR2在MSCs调控NSCs增殖与分化过程中可能起重要作用.

Mesenchymal stem cells regulate the proliferation and differentiation of neural stem cells through vascular endothelial growth factor receptor 2

Objective To study the effect of vascular endothelial growth factor receptor 2 (VEG-FR2) on proliferation and differentiation of neural stem cells (NSCs) regulated by mesenchymal stem cells (MSCs). Methods In co-culture experiments, NSCs were co-cultured with MSCs, human microvascular endothelial ceils (HMVECs) or NIH3T3. To exclude the effect of VEGFR2 ,SU5416 (a selective inhibitor of the tyrosine kinase activity of the VEGFR2) was applied in co-culture system. Six h later,the expression of nestin and GFAP was detected by RT-PCR. Results RT-PCR studies revealed that the expression of nestin in NSCs was up-regulated by MSCs and HMVECs, whereas the level was sharply down-regulated when VEGFR2 was blocked,and the expression of GFAP was increased. Determination of Notchl and Hesl in NSCs showed that MSCs and HMVECs promoted the expression of Notchl and Hesl. Conclusion VEGFR2 may play an important role in the proliferation and differentiation of NSCs regulated by MSCs.