真皮下血管网岛状皮瓣抗菌力的实验研究

为研究真皮下血管网薄皮瓣用于感染创面的可行性，在家猪臀部两侧分别形成以旋髂深血管主干分支为蒂的传统岛状皮瓣和远侧５０％修薄的真皮下血管网岛状皮瓣。用细菌计数、吖啶橙荧光染色测定白细胞吞噬指数和白细胞内杀菌率，应用激光多普勒、墨汁灌注、透明标本等方法，对两种皮瓣的抗菌力作自身对照研究。结果表明，真皮下血管网岛状皮瓣的抗菌力明显降低，可能与皮瓣修薄后血供减少，白细胞功能相应降低有关。

Experimental study on antibacterial activity of subdermal vascular network island skin flap.

A comparison study was carried out on the antibacterial activity between two kinds of pig buttock flaps :the conventional island skin flap and the subdermal vascular network island skin flap which was created by thinning the distal half of a conventional island flap,preserving its subdermal vascular network. Antibacterial activity was evaluated by bacterial counts,phagocytic index and intracellular bacterial killing ratio of leukocytes, skin temperature, laser Doppler,ink perfusion,microangiography and transparent specimen technique. The results indicated that antibacterial activity of the subdermal vascu lar network island skin flap was much lower than that of the conventional island skin flap. The reduced blood supply and decreased leukocyte function were responsible for the low antibacterial activity after the conventional island flap was thinned.