| 人ZP3融合蛋白的原核表达 |
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| 引用本文： | 郭焱,邹冬辉,左文静,路英丽,李丹地,王忠山.人ZP3融合蛋白的原核表达[J].中国妇幼保健,2006,21(6):820-822. |
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| 作者姓名： | 郭焱 邹冬辉 左文静 路英丽 李丹地 王忠山 |
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| 作者单位： | 吉林大学基础医学院细胞生物教研室,130021 |
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| 基金项目： | 国家自然科学基金资助项目(NO.39870313) |
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| 摘 要： | 目的:在原核中表达人卵透明带蛋白3(zone pelluc ida,ZP3)。方法:将人ZP3基因的核心片段克隆到原核表达载体pGEX-4T-1中。经酶切和序列分析后,用重组质粒转化大肠杆菌BL21,并经丙基β-D硫代半乳糖苷(IPTG)诱导产生GST-ZP3融合蛋白。结果:重组菌株明显诱导表达出预期分子质量为63 000 D的融合蛋白。结论:成功地构建GST-ZP3融合蛋白表达载体,并在大肠杆菌中表达GST-ZP3融合蛋白,为进一步开展免疫避孕与生殖免疫的研究奠定基础。
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| 关 键 词： | 卵透明带蛋白3 融合蛋白 原核表达 |
| 文章编号： | 1001-4411（2006）06-0820-03 |
| 收稿时间： | 2005-04-06 |
| 修稿时间： | 2005-04-06 |
Expression of ZP3 fusion protein in prokaryotic cells |
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| GUO Yan, ZHOU Dong - Hui, ZHUO Wen - Jing,et al..Expression of ZP3 fusion protein in prokaryotic cells[J].Maternal and Child Health Care of China,2006,21(6):820-822. |
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| Authors: | GUO Yan ZHOU Dong - Hui ZHUO Wen - Jing |
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| Institution: | Department of Cell Biology School of Basic Medical Sciences, Jiling University, Changchun 130021, Jiling, China |
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| Abstract: | Objective:To express zone pellucida3(zp3) in prokaryotic cells.Methods:The core fragment of zp3 gene was cloned into plasmid pGEX-4T-1 containing glutathiones transferase(GST) fusion protein gene.Following restriction enzyme digestion analysis and sequencing,pGEX-4T-1ZP3 was transformed into E.coli BL21.GST-ZP3 fusion protein was expressed under IPTG induction.Results:A 63 000 Dalton fusion protein was detected,as expected,was obtained evidently.Conclusion:A prokaryotic system capable of expressing GST-ZP3 fusion protein is successfully constructed.This will facilitate our study of the immunocontraception. |
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| Keywords: | Zone pellucida 3(ZP3) Fusion protein Prokaryotic expression |
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