| 中耳胆脂瘤组织中的Ras蛋白的表达及病理意义 |
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| 引用本文： | 黄之勤. 中耳胆脂瘤组织中的Ras蛋白的表达及病理意义[J]. 中国中西医结合耳鼻咽喉科杂志, 2008, 16(5): 328-330 |
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| 作者姓名： | 黄之勤 |
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| 作者单位： | 中南财经政法大学医院耳鼻咽喉科,武汉,430074 |
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| 摘 要： | 目的观察Ras蛋白在继发性中耳胆脂瘤组织中的表达活性及其病理意义。方法32例胆脂瘤上皮标本和12例正常面部表皮标本，免疫组织化学法（SP法）分别检NRas蛋白表达水平，并应用HPIAL-2000高分辨图象分析系统进行半定量分析，比较不同组织中的Ras活性表达差异。结果Ras蛋白的免疫阳性染色物呈棕黄色颗粒。在胆脂瘤上皮细胞，阳性反应物位于胞膜者28例（28／32）；同时位于胞膜、胞浆者2例（2／32）；仅存在于胞浆者2例（2／32）。在正常表皮细胞，阳性反应物主要位于胞膜，以基底层细胞着色为主。两组的阳性反应物平均吸光度（A值）分别为0．826和0．438，组间差异具有统计学意义（P〈0．001）。结论继发性中耳胆脂瘤上皮组织中Ras蛋白的表达活性明显升高，提示胆脂瘤上皮具有高度的增殖活性。
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| 关 键 词： | 中耳胆脂瘤 RAS蛋白 免疫组织化学 表达活性 病理意义 |
The expressive activity of Ras Protein in the epithelia of human middle ear cholesteatoma and its implication in the pathogenesis of the lesion |
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| HUANG Zhiqin. The expressive activity of Ras Protein in the epithelia of human middle ear cholesteatoma and its implication in the pathogenesis of the lesion[J]. Chinese Journal of Otorhinolaryngology of Integrated Traditional and Western Medicine, 2008, 16(5): 328-330 |
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| Authors: | HUANG Zhiqin |
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| Affiliation: | HUANG Zhiqin Dept.of Otolaryngology,the Hospital of Southern Central University of Economics , Law,Wuhan,430074,China |
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| Abstract: | Objective To investigate the expressive activity of Ras protein in the epithelia of secondary human middle ear cholesteatoma and its implication in the pathogenesis of the lesion based on trial carried out in a group of clinical samples.Methods Included in this study were 32 epithelial samples of human middle ear cholesteatoma,with 12 facial skin samples of healthy human as controls.Immunohistochemical SP procedures were taken to detect the expressive activity of Ras protein in these samples,with HPIAL-2000... |
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| Keywords: | Middle ear cholesteatoma Ras protein Immunohistochemistry Expressive activity Pathological implications |
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