| HBV基因型分布与慢性乙肝临床关系的研究 |
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| 引用本文： | 王其亮,李旭. HBV基因型分布与慢性乙肝临床关系的研究[J]. 安徽医药, 2010, 14(7): 781-783. DOI: 10.3969/j.issn.1009-6469.2010.07.018 |
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| 作者姓名： | 王其亮 李旭 |
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| 作者单位： | 安徽医科大学第一附属医院感染病科,安徽,合肥,230022;安徽省濉溪县人民医院感染病科,安徽,淮北,235100;安徽医科大学第一附属医院感染病科,安徽,合肥,230022 |
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| 摘 要： | 目的了解淮北市慢性乙型肝炎患者血清中乙型肝炎病毒（HBV）基因型的分布情况,HBV基因型与临床的关系。方法选择HBV-DNA阳性的慢性乙型肝炎（71例）和肝硬化（22例）患者血清93份,采用巢式PCR法检测HBV基因型。结果本组患者HBV基因分型结果为C型57例（61.3%）,B型16例（17.2%）,B/C混合型20例（21.5%）;C、B和B/C基因型HBV分布在性别上无统计学差异;在慢性乙型肝炎和肝硬化两组中C型比例分别为66.2%,45.5%,差异无有统计学意义（P〉0.05）;C、B和B/C基因型患者HBeAg阳性率分别为79.0%（45/57）,68.8%（11/16）,65.0%（13/20）,差异均无统计学意义（P〉0.05）。B/C基因型与B型和C型基因患者在HBV-DNA和ALT水平上有显著统计学意义（P〈0.01）。结论淮北地区HBV优势基因型以C型为主,B/C混合型次之,有少量的B基因型,B/C混合基因型HBV在病毒复制和肝脏损害方面较B型和C型严重。
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| 关 键 词： | 乙型肝炎 乙型肝炎病毒基因型 特异性引物巢式PCR |
Detection for genotypes of hepatitis B virus by nested PCR with primers and its clinical relevance |
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| WANG Qi-liang,LI Xu. Detection for genotypes of hepatitis B virus by nested PCR with primers and its clinical relevance[J]. Anhui Medical and Pharmaceutical Journal, 2010, 14(7): 781-783. DOI: 10.3969/j.issn.1009-6469.2010.07.018 |
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| Authors: | WANG Qi-liang LI Xu |
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| Affiliation: | WANG Qi-liang1,2,LI Xu1(1.Department of Infectius Diseases,First Affiliated Hospital,Anhui Medicai University,Hefei 230022,China2.Suixi Country People′s Hospital,Huaibei 235100) |
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| Abstract: | Aim To determine the distribution of hepatitis B virus(HBV)genotypes in patients with chronic infection in Huaibei city,Anhui province of China by nested PCR with genotype-specific primers.Methods 93 serum samples from HBV-DNA Positive patients with chronic hepatitis B virus infection were divided into 2 groups according to patients' diagnosis.The 2 groups were chronic hepatitis B and cirrhosis respectively.Nested PCR with genotype-specific primers was used to determinate the genotypes of HBV.Results Of the... |
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| Keywords: | hepatitis B virus hepatitis B viral genotype nested PCR genotype-specific primers |
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