分泌型卷曲相关蛋白2基因超甲基化与大肠癌的研究

目的:研究分泌型卷曲相关蛋白2(SFRP2)基因启动子超甲基化与大肠癌的关系.方法:用甲基化荧光定量PCR(MethyLight)技术检测44例散发型大肠癌和20例癌旁组织中SFRP2基因的启动子甲基化状况,20例健康志愿者大肠组织作为对照组.同时分析SFRP2基因超甲基化与临床病理特征的关系.结果:44例大肠癌组织中检出SFRP2基因超甲基化36例(36/44,81.8%),20例大肠癌旁组织中检出SFRP2基因超甲基化8例(8/20,40%),20例正常大肠黏膜组织中没有显示SFRP2基因任何甲基化条带.大肠癌中SFRP2基因甲基化较正常黏膜和邻近的正常黏膜更频繁.并且SFRP2基因超甲基化与任何包括性别、年龄、肿瘤分期、位置、组织学分级等临床病理特征之间没有显著的关联.结论:SFRP2基因超甲基化与大肠癌的发生有关,SFRP2基因超甲基化可以作为大肠癌筛检的高潜力标记.

A study on relation of hypermethylation of secreted frizzled-related protein 2 (SFRP2) gene with colorectal cancer

Objective:To investigate the function of promoter hypermethylation of secreted frizzled-related protein 2(SFRP2) gene in colorectal cancer.Methods:The promoter hypermethylation of SFRP2 gene in 44 sporadic colorectal cancer tissues and 20 adjacent normal mucosas of colorectal cancers were detected by fluorescence-based real-time PCR assay(MethyLight),using 20 colorectal tissues of healthy volunteer as controls.And the relationship between hypermethylation of SFRP2 gene and clinicopathological features were analyzed.Results:SFRP2 gene hypermethylation was found in 81.8%(36/44)colorectal cancer tissues and 40%(8/20)adjacent normal mucosas of colorectal cancer.None of the normal colorectal mucosa tissues of volunteers showed any hypermethylated bands of SFRP2.Methylation in colorectal tumors was more frequent than in normal mucosa and adjacent tumor normal mucosa.Moreover,no significant associations were observed between SFRP2 hypermethylation and clinicopathological features such as sex,age,tumor stage,location and histological grade.Conclusion:Hypermethylation of SFRP2 gene is associated with the development of colorectal cancer.Hypermethylation of SFRP2 gene might serve as a biomarker for colorectal cancer screening.