神经性钙粘蛋白在肝门部胆管癌中的表达及意义

【摘要】目的 研究神经型钙黏蛋白（N-cadherin）在肝门部胆管癌组织中的表达及临床意义。方法 免疫组化检测26例肝门部胆管癌与癌旁及5例正常胆管组织中N-cadherin表达差异。Western blot对比N-cadherin在肝门部胆管癌组织中与癌旁中差异。结果 免疫组化示26例肝门部胆管癌癌组织中有14例N-cadherin表达阳性，癌旁组织中6例阳性（P<0.05）。正常胆管阳性表达0例。N-cadherin在低分化癌组阳性表达率高于中、高分化癌组。 Western blot示N-cadherin在肝门部胆管癌组织中的表达高于癌旁组织(0.88±0.12vs0.43±0.09,P<0.05)。结论 Ncadherin可能参与肝门部胆管癌的发生发展。

Expression and Significance of Neural Cadherin in Perihilar Cholangiocarcinoma

[Abstract] Objective To explore the expression and the clinical significance of neural cadherin (N- cadherin) in perihilar cholangiocarcinoma.Methods The expression of N- cadherin was detected by immunohistochemical method in 26patients with perihilar cholangiocarcinoma and5samples of normal bile duct tissues. The expression of N-cadherin was also compared by Western blotting assay between perihilar cholangiocarcinoma and paraneoplastic tissues.Results The im? munohistochemical results showed that positive expression of N-cadherin was observed in14of26patients with perihilar cholangiocarcinoma and in6of26paraneoplastic samples (53.84%vs23.08%,P<0.05). There was 0N-cadherin expres? sion in normal bile duct tissues. The positive rate of N-cadherin expression was higher in low differentiated group than that in middle and well differentiated groups. The result of Western blotting assay showed that N-cadherin expression was signifi? cantly higher in perihilar cholangiocarcinoma than that in the paraneoplastic tissues (0.88±0.12vs0.43±0.09, P<0.05) Conclusion N-cadherin may be involved in the occurrence and development of perihilar cholangiocarcinoma.