脑白质损伤新生大鼠VEGF及iNOS表达变化研究

目的 研究缺氧缺血(HI)对新生大鼠脑白质细胞血管内皮生长因子(VEGF)及诱导型一氧化氮合酶(iNOS)表达的影响,探讨VEGF及iNOS在新生大鼠脑白质损伤(WMD)发病机制中的作用。方法 将3日龄大鼠随机分为实验组及假手术组,建立新生大鼠WMD模型,分别予HI后12、24、48、72 h及7 d处死,对其脑组织取材后行HE染色观察其病理改变,并应用免疫组织化学法检测其VEGF及iNOS的表达水平。结果 在新生大鼠脑白质中,1)VEGF表达水平予HI后12 h开始明显上升,24 h后达高峰,至7 d恢复正常,与对照组比较,实验组脑室周围白质VEGF的表达在12、24、48、72 h有统计学意义。2)实验组iNOS于HI后12 h表达开始增加,72 h达高峰,7 d仍有表达,与对照组比较,差异有统计学意义。 结论 HI可导致新生大鼠脑白质VEGF及iNOS的表达水平显著增高,参与新生大鼠WMD的病理生理过程。

Changes in expressions of vascular endothelial growth factor and inducible nitric oxide synthase protein in neonatal rats with white matter damage.

Objective To observe the expressions of vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase(iNOS) protein in the brains of premature rats to moderate hypoxia-ischemia (HI),in order to explore possible relationships with white matter damage(WMD). Methods The 3 days old rats were randomly divided into experiment group and sham operation group.The pups were perfused at 12,24,48,72 h and 7 d of recovery from HI.Immunohistochemical technical was applied to investigate the changes of expressions of VEGF and iNOS in periventricular rats white matter tissues. Results In the experiment group,the expression of VEGF in periventricular rats white matter started to increase at 12 h and reached the peak at 24 h.The expression of iNOS was up-regulated at 12 h and peaked at 72 h after HI. Conclusion HI could induce the prominent increase in the expressions of VEGF and iNOS in white matter of the neonatal rats' brain,and may be involved in the pathophysiological process in the WMD.