Expression of 4-hydroxynonenal in the kidney of diabetic rats and the effect of probucol

Objective To investigate the expression of 4-hydroxynonenal (4-HNE) in the kidney of diabetic rats and the effect of probucol. Methods The rats were being intraperitoneal injected with STZ (60 mg/kg) to establish diabetic models. Then diabetic rats were randomly divided into diabetic group (group D, n＝24), probucol treated group (group P, n＝24). Normal rats were taken as control group (group C, n＝24). Rats in group P were treated by probucol (110 mg·kg-1·d-1); rats in group D and group C were given equal volume water instead. Scr, BUN, triglyceride (TG), total cholesterol (TC) and 24-hour urinary proteinin were measured at the 4th, 8th and 12th week. PAS staining and HE staining were used to evaluate the pathological changes of the kidney. The immunohistochemistry and Western blotting were used to detect the expression of 4-HNE in renal tissue. Results Levels of Scr, BUN, TG, TC and 24-hour urinary protein in group D were higher than those in group C at the 4th, 8th and 12th week(all P＜0.05); Levels of Scr, BUN, TG, TC and 24-huor urinary protein in group P were lower than those in group D at 4th, 8th and 12th week (all P＜0.05). The pathological changes of the kidney in group D were more serious than that in group P. The expression of 4-HNE in group Dwerehigher than group C at the 4th, 8th and 12th week (all P＜0.05); The expression of 4-HNE in the kidneys of group P decreased significantly compared to that of group D at the same time (P＜0.05). Conclusions As an indicator of lipid peroxidation, the expression of 4-HNE significantly increases in the kidney of diabetic rat. Probucol may protect the diabetic kidney through decreasing the expression of 4-HNE and the level of lipidperoxidation.