顺铂对结直肠癌细胞增殖及侵袭能力的影响

【摘要】目的 探讨顺铂对人结直肠癌细胞SW480增殖及侵袭力的影响。方法 以SW480细胞为研究对象，设定未经处理的SW480细胞为对照组，给予不同浓度顺铂进行不同时间干预，应用MTT法、Transwell侵袭小室、定磷法检测SW480细胞的增殖、侵袭力及Na + -K + -ATP酶活性。结果 生理浓度顺铂（70μmol/L）在48h即可抑制SW480 细胞的增殖，与72和96h相比抑制率差异无统计学意义；70μmol/L顺铂处理48h时，即可降低穿越Matrigel膜基质的细胞数；分别采用17.5、35、70和140µmol/L4个梯度浓度的顺铂作用于SW480细胞48h后，35、70和140µmol/L组细胞中Na + -K + -ATP酶活性均显著升高，且顺铂浓度达70µmol/L时Na + -K + -ATP酶即可达到较高活性。结论 Na + - K + -ATP酶活性的降低可能导致对结直肠癌细胞增殖和侵袭能力调节作用减弱，可能与结直肠癌细胞SW480耐顺铂有关。

Effects of Cisplatin on Proliferation and Invasion of Colorectal Cancer Cells

Abstract] Objective To investigate the effect of cisplatin on proliferation and invasion of colorectal cancer cell line SW480. Methods SW480cells were used as the research object, and untreated SW480cells were used as the control group. Different concentrations of cisplatin was given at different times to intervene. The MTT, Transwell chamber and phos? phate determination methods were used to detect proliferation, invasion and Na + - K + - ATPase activity expression level in SW480cells.Results The physiological concentration of cisplatin (70µmol/L) inhibited the proliferation of SW480cells at 48h. There was no significant difference in the inhibition rate at48h compared with72h and96h. Treatment with70 µmol/L cisplatin for48h reduced the number of cells through Matrigel membrane matrix. The Na + -K + -ATPase activity was significantly increased in 35, 70and140µmol/L cells after treatment with17.5, 35, 70and140µmol/L of cisplatin in SW480cells for48h, and Na + -K + -ATPase reached the highest level at70µmol/L of cisplatin.Conclusion The decreased activity of Na + -K + -ATPase may lead to the attenuation in proliferation and invasion of colorectal cancer cells, which may be associated with cisplatin resistance in colorectal cancer cell SW480.