镁离子对大鼠血管平滑肌细胞钙化的影响 , 镁离子对大鼠血管平滑肌细胞钙化的影响

目的 探讨不同浓度镁离子对大鼠血管平滑肌细胞(VSMCs)钙化的影响。方法 原代培养获取 VSMCs，进行形态学及免疫细胞鉴定，后将VSMCs随机分为阴性对照组、高磷组、镁干预组。阴性对照组采用含10%胎牛血清培养，高磷组采用高磷培养基培养，镁干预组在高磷培养基的基础上分别加入不同浓度氯化镁，使镁离子终浓度分别为1、2、3 mmol/L（镁干预组1~3），刺激7 d后行钙化检测，测定钙含量及碱性磷酸酶（ALP）活性，并行反转录聚合酶链反应（RT-PCR）检测细胞内核心结合因子α1（Cbfα1）mRNA的表达。结果 高磷组和镁干预组VSMCs均有钙盐沉积，其钙含量均高于阴性对照组；镁干预组随镁离子浓度增大钙化结节逐渐缩小，除镁干预组1钙含量与高磷组无差异外，镁干预组2和镁干预组3均低于高磷组（均P＜0.05）。VSMCs ALP活性和Cbfα1 mRNA的表达除镁干预组3与阴性对照组无差异外，其余组均高于阴性对照组（P＜0.05）。镁干预组随镁离子浓度增大，ALP活性和Cbfα1 mRNA的表达水平均逐渐降低，且均低于高磷组（P＜0.05）。结论 镁离子可在一定程度上抑制高磷诱导的VSMCs钙化和成骨样转分化，其可能是通过降低VSMCs中Cbfα1的表达来实现的。

Effect of magnesium ion on the calcification of vascular smooth muscle cells,Effect of magnesium ion on the calcification of vascular smooth muscle cells

Objective: To explore the effect of the different concentrations of magnesium ions on rat vascular smooth muscle cell (VSMCs) calcification. Methods: VSMCs were obtained from rat aortic, and identified by immunocytochemistry, then randomly divided into control group, high phosphorus group, magnesium intervention group (the group was settled three subgroups according to the concentration of magnesium ions based on based on the high phosphorus medium, namely 1 mmol/L, 2 mmol/L and 3 mmol/L).Calcification staining, calcium content and alkaline phosphatase activity were measured and the expression of Cbfα1 mRNA was detected by RT-PCR after stimulating 7 days. Results: Compared with the control group, the remaining groups had calcium deposits, and they were higher than the control group, the calcified nodules gradually reduced with the increasing magnesium ion concentration in the intervention group, In addition to the calcium content in the intervention group was no difference with the high phosphorus group, the intervention group 2 and 3 were lower than the high phosphate of calcium content (P<0.05), compared with the control group, ALP activity and Cbfα1 mRNA expression were no difference in the intervention group 2, the remaining groups were higher than the negative control group (P<0.05). ALP activity and the expression of Cbfα1 mRNA were gradually decreased with the increasing magnesium ion concentration in the intervention group, and were lower than the high phosphorus group (P<0.05). Conclusion: Magnesium can reduce calci?cation and osteoblastic transdifferentiation in vascular smooth muscle cells in a certain extent, which may be achieved by reducing the expression of Cbfα1 in VSMCs Cbfα1.