| 慢性复合应激对大鼠海马生长休止蛋白7表达的影响 |
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| 引用本文： | 廖家万,胡红梅,钟敬亮,孙朝越,孙新明,彭建安,刘胜洪.慢性复合应激对大鼠海马生长休止蛋白7表达的影响[J].解剖学报,2008,39(6):826-830. |
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| 作者姓名： | 廖家万 胡红梅 钟敬亮 孙朝越 孙新明 彭建安 刘胜洪 |
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| 作者单位： | 1.井冈山大学医学院组织学与胚胎学教研室，江西 吉安 343300; 2.华中科技大学同济医学院组织学与胚胎学教研室，武汉 430030 |
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| 基金项目： | 江西省教育厅科技项目
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| 摘 要： | 目的 探讨慢性复合应激对大鼠海马神经元中生长休止蛋白7(Gas7)的表达变化及意义.方法 36只大鼠随机分为慢性复合应激组和正常对照组.复合应激组动物进行6周的垂直旋转、睡眠剥夺、捆绑(6h/d)和夜间光照等慢性复合性应激试验;实验结束后,所有动物采用免疫组织化学和免疫印迹等方法 检测大鼠海马Gas7蛋白表达的变化和神经元凋亡情况.结果 Gas7在对照组和实验组大鼠海马各区均有表达,主要表达在海马神经元的胞质和突起内.其中在CA1区和齿状回呈较强阳性表达,CA3区表达则较弱;慢性复合应激组CA1区和齿状回阳性染色加深,平均吸光度明显上升(P<0.05),而CA3区则不明显(P>0.05).慢性复合应激组中部分切片在下托可见少量Caspase-3免疫反应阳性细胞.免疫印迹检测表明,慢性复合应激组大鼠海马Gas7表达明显增强,与正常对照组相比,差异具有显著性(P<0.05).结论 Gas7可能参与了在应激情况对神经元的保护作用,以及促进神经元的发生和发育.
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| 关 键 词： | 生长休止蛋白7 海马 神经元 慢性复合应激 免疫组织化学 免疫印迹 大鼠 |
| 收稿时间： | 2008-5-5 |
| 修稿时间： | 2008-8-14 |
EFFECT OF CHRONIC MULTIPLE STRESS ON THE EXPRESSION OF GROWTH-ARREST-SPECIFIC PROTEIN 7 IN THE HIPPOCAMPUS OF RATS |
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| LIAO Jia-wan,HU Hong-mei,ZHONG Jing-liang,SUN Chao-yue,SUN Xing-ming,PENG Jian-an,LIU Sheng-hong.EFFECT OF CHRONIC MULTIPLE STRESS ON THE EXPRESSION OF GROWTH-ARREST-SPECIFIC PROTEIN 7 IN THE HIPPOCAMPUS OF RATS[J].Acta Anatomica Sinica,2008,39(6):826-830. |
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| Authors: | LIAO Jia-wan HU Hong-mei ZHONG Jing-liang SUN Chao-yue SUN Xing-ming PENG Jian-an LIU Sheng-hong |
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| Institution: | 1.Department of Histology and Embryology,Medical College of Jinggangshan University，Jiangxi Ji′an 343300, China;2.Department of Histology and Embryology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030，China |
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| Abstract: | Objective To study the expression of growth-arrest-specific protein 7(Gas7) in the hippocampus of rats after chronic multiple-stress.Methods thirty-six Wistar rats were randomly divided into two groups:the chronic multiple stressed group and the control group.Rats in the stressed group were administered with four kinds of stressors including vertical rotation,sleeping deprivation,restraint(6hours/day) and illumination irregularly and alternately for 6 weeks.Then the expression of Gas7 protein in the hippoca... |
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| Keywords: | Gas7 Hippocampus Neuron Chronic multiple-stress Immunohistochemistry Western blotting Rats |
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