穴位贴敷对慢性萎缩性胃炎大鼠胃黏膜血流量、前列腺素E_2的影响

目的:探讨穴位贴敷治疗慢性萎缩性胃炎的作用机理,为临床应用提供科学依据。方法:采用长期喂养0 .0 2 %的氨水诱发大鼠慢性萎缩性胃炎为动物模型,选取“足三里”“中脘”穴进行穴位贴敷治疗,与口服胃苏冲剂进行对照,观察穴位贴敷对慢性萎缩性胃炎大鼠一般状态及体重、胃黏膜血流量、胃黏膜前列腺素E2 的影响。结果:穴位贴敷可以改善大鼠一般状态及体重,增加胃黏膜血流量及胃黏膜前列腺素E2 ,其作用优于药物对照组。结论:穴位贴敷对胃黏膜确有保护作用,是临床治疗慢性萎缩性胃炎的有效方法之一。

Effect of Acupoint Application on Gastric Mucosal Blood Flow and Prostaglandin E2 Content in Rats with Chronic Atrophic Gastritis

Objective:To observe the effect of acupoint application on gastric mucosal blood flow, prostaglandin E_2 content in rats with chronic atrophic gastritis (CAG). Methods:A total of 48 SD rats were evenly randomized into control group, model group, acupoint application “Zusanli” (ST 36) and “Zhongwan”(CV12) group and medication group. CAG model was established by feeding the rat with 0.02% Ammonia solution for 16 weeks. Rats of medication group were fed with “Weisu Chongji” (herbal granule for protecting gastric function, 804 mg/200 g body weight). Acupoint application and gastric perfusion of “Weisu Chongji” were given once daily, continuously for 2 months. Gastric mucosal blood flow (GMBF) and prostaglandin E_2 content (PGE_2) of the gastric mucosal tissue were assayed with neutral red clearance method and radioimmunoassay respectively. Results: In comparison with control group, the rats' body weight, GMBF and gastric mucosal PGE_2 levels of model group were significantly lower (P<0.01); while the body weight and GMBF of acupoint application and medication groups, and gastric mucosal PGE_2 content of acupoint application group were significantly higher than those of model group (P<0.01). Comparison between acupoint application and medication groups showed that the body weight, GMBF and gastric mucosal PGE_2 content of acupoint application group were significantly higher than those of medication group (P<0.05). The facts indicated that acupoint application and medication both could improve gastric mucosal blood flow and CAG rats′ constitution and raise gastric mucosal PGE_2 level and the therapeutic effect of acupoint application was superior or significantly superior to that of medication. Conclusion:Acupoint application can protect gastric mucosa by improving GMBF and increasing PGE_2 in chronic atrophic gastritis rats and is thus one of the effective therapies for CAG in clinic.