胆固醇对血管内皮细胞的损伤

目的 研究胆固醇对人血管内皮细胞的损伤作用.方法 体外培养人脐静脉内皮细胞株ECV304,分别以浓度为6.25、12.50、25.00、50.00 mg/L的胆固醇作用于ECV304细胞48 h,检测细胞培养液中乳酸脱氢酶(lactate dehydrogenase, LDH)活性,一氧化氮(nitric oxide, NO)浓度,一氧化氮合酶(nitric oxide synthase, NOS)活性及单核细胞趋化蛋白-1(MCP-1)浓度.结果 与无胆固醇组比较,胆固醇浓度为50.00 mg/L时培养液中LDH活性显著增加;25.0 mg/L,50.0 mg/L浓度组培养液中NO浓度显著降低;胆固醇浓度为6.25、12.5、25.0、50.0 mg/L组与无胆固醇组比较,随着胆固醇浓度提高,培养液中NOS活性逐渐降低,MCP-1浓度逐渐增高,有显著性差异.结论 一定浓度的胆固醇能直接损伤人血管内皮细胞结构,影响内皮细胞的部分分泌功能.

Study on injury of human vascular endothelial cells by cholesterol

Objective To investigate the injuries caused by cholesterol to the vascular endothelial cells (VECs). Methods Different dosage of cholesterol (6.25,12.5,25.0,50.0 mg/L) was used on human umbilical endothelial cell line,ECV304,respectively. LDH activity,nitric oxide and the nitric oxide synthetase activity in the supernatant of cell culture were detected. The concentration of MCP-1 protein in cell culture was detected by ELISA. Results As compared with the normal control cells,a significant increase of LDH activity was found in the cells treated with 50.0 mg/L cholesterol. The NO level decreased in the cells treated by 25.0 or 50.0 mg/L cholesterol. When treated by cholesterol at dose of 6.25,12.5,25.0 or 50.0 mg/L respectively,the NOS activity was greatly decreased and MCP-1 protein was significantly increased in a dose-dependent manner. Conclusion Cholesterol of high concentration could directly injure the structure and partial function of VECs.